Barakat Ashraf M A, El-Razik Khaled A Abd, Elfadaly Hassan A, Rabie Nagwa S, Sadek Sabry A S, Almuzaini Abdulaziz M
Department of Zoonotic Diseases, National Research Centre, Dokki, Giza, Egypt.
Department of Animal Reproduction, National Research Centre, Dokki, Giza, Egypt.
Vet World. 2020 Jul;13(7):1430-1438. doi: 10.14202/vetworld.2020.1430-1438. Epub 2020 Jul 24.
Campylobacteriosis is one of the most well-characterized bacterial foodborne infections worldwide that arise chiefly due to the consumption of foods of animal origin such as poultry, milk, and their products. The disease is caused by numerous species within the genus , but is the most commonly isolated species from established cases of human campylobacteriosis. This study was conducted to determine the prevalence and virulence of isolates from human, chicken, and milk and milk products in Egypt.
A total of 1299 samples (547 chicken intestine and liver, 647 milk and milk products, and 105 human stool) were collected and microbiologically investigated, confirmed by multiplex polymerase chain reaction (PCR) targeting the 23S rRNA, , and genes specific for spp., , and , respectively, followed by virulence genes ( adhesion to fibronectin F [] and ) detection using PCR.
About 38.09%, 37.84%, and 8.5% of human stool, chicken, and milk and milk product samples, respectively, were bacteriologically positive, with a total of 302 isolates. All isolates were molecularly confirmed as spp. (100%) where 285 isolates (94.37%) were identified as and 17 isolates (5.62%) as . Regarding the virulence pattern, all isolates (100%) carried gene while cytolethal distending toxin B gene was definite in 284/302 isolates (94%), concisely, 282/285 (98.94%) isolates, and in 2/17 (11.76%) isolates.
The widespread presence of these highly virulent , especially , proofs the urgent need for the implementation of stringent control, public health, and food protection strategies to protect consumers from this zoonotic pathogen. The availability of information about pathogen virulence will enable enhanced local policy drafting by food safety and public health officials.
弯曲菌病是全球特征最明确的细菌性食源性感染之一，主要因食用如家禽、牛奶及其制品等动物源性食品而引发。该病由弯曲菌属内的众多菌种引起，但空肠弯曲菌是人类弯曲菌病确诊病例中最常分离出的菌种。本研究旨在确定埃及人、鸡肉以及牛奶和奶制品中分离出的空肠弯曲菌的流行情况和毒力。
共收集了1299份样本（547份鸡肠道和肝脏样本、647份牛奶和奶制品样本以及105份人类粪便样本）并进行微生物学调查，通过针对空肠弯曲菌属、空肠弯曲菌和结肠弯曲菌特异性的23S rRNA、空肠弯曲菌基因和结肠弯曲菌基因的多重聚合酶链反应（PCR）进行确认，随后使用PCR检测毒力基因（对纤连蛋白F的黏附力[flaA]和cadF）。
分别约有38.09%、37.84%和8.5%的人类粪便、鸡肉以及牛奶和奶制品样本细菌学检测呈阳性，共分离出302株空肠弯曲菌。所有分离株经分子学确认均为空肠弯曲菌属（100%），其中285株（94.37%）被鉴定为空肠弯曲菌，17株（5.62%）为结肠弯曲菌。关于毒力模式，所有分离株（100%）携带flaA基因，而细胞致死膨胀毒素B基因在284/302株分离株（94%）中确定，具体而言，在282/285株（98.94%）空肠弯曲菌分离株以及2/17株（11.76%）结肠弯曲菌分离株中存在。
这些高毒力的空肠弯曲菌，尤其是空肠弯曲菌的广泛存在，证明迫切需要实施严格的控制、公共卫生和食品保护策略，以保护消费者免受这种人畜共患病原体的侵害。病原体毒力信息的可得性将有助于食品安全和公共卫生官员加强地方政策的制定。
