Olaru Octavian Tudorel, Venables Luanne, VAN DE Venter Maryna, Nitulescu George Mihai, Margina Denisa, Spandidos Demetrios A, Tsatsakis Aristidis M
Faculty of Pharmacy, 'Carol Davila' University of Medicine and Pharmacy, Bucharest 020956, Romania.
Department of Biochemistry and Microbiology, Nelson Mandela Metropolitan University, Port Elizabeth 6031, South Africa.
Oncol Lett. 2015 Sep;10(3):1323-1332. doi: 10.3892/ol.2015.3453. Epub 2015 Jul 2.
The aim of the present study was to determine the anticancer potential of three species belonging to the genus (Polygonaceae): (, () and (). For this purpose, crude extracts were obtained and characterized for their phenolic and flavonoid total content and examined for their anticancer activity on three tumor cell lines: breast cancer (MCF7), colon carcinoma (Caco-2) and cervical cancer (HeLa) cells. The cytotoxic potential of the three species was assessed by MTT assay, cell cycle analysis and by the evaluation of mitochondrial membrane potential (MMP). The acute toxicity of the extracts was evaluated using one cell model (Vero cells, an African Green monkey kidney cell line) and two invertebrate models ( and ). The highest total phenolic and flavonoid content was found in the flower extracts. The cytotoxic effects of the extracts from and on all three cell lines were examined at concentrations ranging from 3 to 300 µg/ml. G2/M cell cycle arrest was induced by all the extracts, and a significant increase in the subG1 cell population was observed. The hydroethanolic extract from the flowers of induced cell apoptosis more rapidly than the other extracts. The MMP indicates the involvement of the mitochondria in the induction of apoptosis. A positive correlation between the total phenolic content of the extracts and the IC values against the HeLa cells was also noted. None of the extracts exhibited significantly toxic effects. Considering the antitumor potential of and , these two species may represent a good source of plant extracts with anticancer properties.
本研究的目的是确定蓼科蓼属三种植物（[植物名称1]、[植物名称2]和[植物名称3]）的抗癌潜力。为此，提取了粗提物并对其总酚和总黄酮含量进行了表征，并检测了它们对三种肿瘤细胞系的抗癌活性：乳腺癌（MCF7）、结肠癌（Caco-2）和宫颈癌（HeLa）细胞。通过MTT法、细胞周期分析和线粒体膜电位（MMP）评估来测定这三种植物的细胞毒性潜力。使用一种细胞模型（Vero细胞，一种非洲绿猴肾细胞系）和两种无脊椎动物模型（[动物模型1]和[动物模型2]）评估提取物的急性毒性。发现[植物名称3]花提取物中的总酚和总黄酮含量最高。检测了[植物名称1]和[植物名称2]提取物在3至300μg/ml浓度范围内对所有三种细胞系的细胞毒性作用。所有提取物均诱导G2/M期细胞周期阻滞，并观察到subG1期细胞群体显著增加。[植物名称3]花的水乙醇提取物比其他提取物更快地诱导细胞凋亡。MMP表明线粒体参与了细胞凋亡的诱导。还注意到提取物的总酚含量与对HeLa细胞的IC值之间呈正相关。没有一种提取物表现出明显的毒性作用。考虑到[植物名称1]和[植物名称2]的抗肿瘤潜力，这两个物种可能是具有抗癌特性的植物提取物的良好来源。
