Naddaf H, Varzi H Najafzade, Sabiza S, Falah H
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Open Vet J. 2014;4(2):85-9. Epub 2014 Sep 17.
This study was designed to evaluate effects of xylazine-ketamine anesthesia on plasma levels of cortisol and vital signs during and after laparotomy in dogs. Eight clinically healthy, adult male dogs, weighing 20 kg were used. All dogs were initially sedated by acepromazine. Thirty minutes later, ketamine plus xylazine was used to induce anesthesia. Surgical incision of laparotomy was done. After a 5 min manipulation of the abdominal organs, the incision was sutured. Vital signs including heart rate, respiratory rate and rectal temperature (RT) were recorded at the times of -30: premedication, 0: induction and Surgical incision, 30: End of surgery, 60, 90 and 120 min. Blood was sampled at the above mentioned times and analyzed using a commercial ELISA kit for cortisol. A significant decreasing trend in RT was observed during the studied times. No significant changes were observed in heart rate and respiratory rate (p>0.05), except at the time of 60 respiratory rate significantly decreased when compared to the time of 90 (p=0.026) and 120 (p=0.041). A non-significant but increasing trend in plasma levels of cortisol was observed.
本研究旨在评估赛拉嗪-氯胺酮麻醉对犬剖腹术期间及术后血浆皮质醇水平和生命体征的影响。使用了8只体重20千克的临床健康成年雄性犬。所有犬最初均用乙酰丙嗪镇静。30分钟后，使用氯胺酮加赛拉嗪诱导麻醉。进行剖腹手术切口。对腹腔器官进行5分钟操作后，缝合切口。在-30（术前用药）、0（诱导和手术切口）、30（手术结束）、60、90和120分钟时记录包括心率、呼吸频率和直肠温度（RT）在内的生命体征。在上述时间采集血液样本，并使用商用ELISA试剂盒分析皮质醇。在研究期间观察到RT有显著下降趋势。除60分钟时呼吸频率与90分钟（p = 0.026）和120分钟（p = 0.041）相比显著降低外，心率和呼吸频率未见显著变化（p>0.05）。观察到血浆皮质醇水平有非显著但上升的趋势。
