Marcher Ann-Britt, Loft Anne, Nielsen Ronni, Vihervaara Terhi, Madsen Jesper Grud Skat, Sysi-Aho Marko, Ekroos Kim, Mandrup Susanne
Department of Biochemistry and Molecular Biology, University of Southern Denmark, 5230 Odense M, Denmark.
Zora Biosciences Oy, Biologinkuja 1, 02150 Espoo, Finland.
Cell Rep. 2015 Dec 1;13(9):2000-13. doi: 10.1016/j.celrep.2015.10.069. Epub 2015 Nov 25.
Cold exposure greatly alters brown adipose tissue (BAT) gene expression and metabolism to increase thermogenic capacity. Here, we used RNA sequencing and mass-spectrometry-based lipidomics to provide a comprehensive resource describing the molecular signature of cold adaptation at the level of the transcriptome and lipidome. We show that short-term (3-day) cold exposure leads to a robust increase in expression of several brown adipocyte genes related to thermogenesis as well as the gene encoding the hormone irisin. However, pathway analysis shows that the most significantly induced genes are those involved in glycerophospholipid synthesis and fatty acid elongation. This is accompanied by significant changes in the acyl chain composition of triacylglycerols (TAGs) as well as subspecies-selective changes of acyl chains in glycerophospholipids. These results indicate that cold adaptation of BAT is associated with significant and highly species-selective remodeling of both TAGs and glycerophospholipids.
冷暴露会极大地改变棕色脂肪组织（BAT）的基因表达和代谢，以提高产热能力。在此，我们使用基于RNA测序和质谱的脂质组学技术，提供了一份全面的资源，描述了转录组和脂质组水平上冷适应的分子特征。我们发现，短期（3天）冷暴露会导致与产热相关的几个棕色脂肪细胞基因以及编码鸢尾素的基因表达显著增加。然而，通路分析表明，诱导最显著的基因是那些参与甘油磷脂合成和脂肪酸延长的基因。这伴随着三酰甘油（TAGs）的酰基链组成以及甘油磷脂中酰基链的亚类选择性变化的显著改变。这些结果表明，BAT的冷适应与TAGs和甘油磷脂的显著且高度物种选择性重塑有关。
