Mukhopadhyay Bedabrata, Gongopadhyay Ajay Narayan, Rani Anjali, Gavel Roshni, Mishra Surendra Pratap
Department of Biochemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
Department of Pediatric Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
J Indian Assoc Pediatr Surg. 2015 Oct-Dec;20(4):179-83. doi: 10.4103/0971-9261.161037.
Several studies using animal models have shown that oxidative stress during pregnancy may play an important role in causing birth defects. Congenital anomalies affect an estimated 270,000 newborns who die during the first 28 days of life every year from different birth defects. Hence, at present many research works are going on to reduce the infant mortality from congenital anomaly.[1].
The objective was to measure the oxidant and antioxidant level in the serum of newborn babies with the congenital anomaly and compare these levels with age and sex matched normal neonates. This is to identify any role of oxidative stress in the causation of congenital anomaly.
This case-control study included 159 participants: 106 newborns with the congenital anomaly and 53 healthy newborns. The markers of oxidative stress like serum malondialdehyde (MDA) level, protein carbonyl (PC) level, and the activity of antioxidants such as Vitamin C, glutathione were measured in both cases (neonates with congenital anomaly) and controls (normal healthy neonates). These parameters were statistically compared.
MDA levels and PC levels were significantly higher (P < 0.0001), and Vitamin C and reduced glutathione levels were significantly lower (P < 0.0001), in newborns with congenital malformation than in healthy newborns.
Increased lipid peroxidation and protein carbonylation might play an important role in the pathogenesis of congenital anomaly. Impairment of the free radical/antioxidant balance is leading to increased free radical damage in neonates with congenital malformation.
多项使用动物模型的研究表明，孕期氧化应激可能在导致出生缺陷方面发挥重要作用。据估计，每年有270,000名新生儿因各种出生缺陷在出生后的头28天内死亡。因此，目前许多研究工作正在进行，以降低先天性异常导致的婴儿死亡率。[1]
目的是测量患有先天性异常的新生儿血清中的氧化剂和抗氧化剂水平，并将这些水平与年龄和性别匹配的正常新生儿进行比较。这是为了确定氧化应激在先天性异常病因中的任何作用。
这项病例对照研究包括159名参与者：106名患有先天性异常的新生儿和53名健康新生儿。在病例组（患有先天性异常的新生儿）和对照组（正常健康新生儿）中均测量了氧化应激标志物，如血清丙二醛（MDA）水平、蛋白质羰基（PC）水平，以及抗氧化剂如维生素C、谷胱甘肽的活性。对这些参数进行了统计学比较。
患有先天性畸形的新生儿的MDA水平和PC水平显著更高（P < 0.0001），而维生素C和还原型谷胱甘肽水平显著更低（P < 0.000），与健康新生儿相比。
脂质过氧化和蛋白质羰基化增加可能在先天性异常的发病机制中起重要作用。自由基/抗氧化剂平衡的损害导致患有先天性畸形的新生儿自由基损伤增加。
