在识别螺旋中具有双交换或三交换的乳糖阻遏物突变体与具有多个交换的乳糖操纵基因变体特异性结合。
lac repressor mutants with double or triple exchanges in the recognition helix bind specifically to lac operator variants with multiple exchanges.
作者信息
Sartorius J, Lehming N, Kisters B, von Wilcken-Bergmann B, Müller-Hill B
机构信息
Institut für Genetik der Universität zu Köln, FRG.
出版信息
EMBO J. 1989 Apr;8(4):1265-70. doi: 10.1002/j.1460-2075.1989.tb03500.x.
Abstract
Several lac repressor mutants have been isolated which repress beta-galactosidase synthesis in Escherichia coli up to 200-fold. They do so by binding specifically to particular symmetrical lac Oc operator variants. The mutations in the lac repressor are localized in two separate parts of the recognition helix comprising (i) residues 1 and 2 which interact with base pairs 4 and 5 of lac operator and (ii) residue 6 which recognizes operator base pair 6. Mutations of residues 1 and 2 may be combined with a mutation of residue 6. The resulting mutant protein binds specifically to an operator variant with three symmetric exchanges in base pairs 4, 5 and 6.
摘要
已经分离出几种乳糖阻遏物突变体，它们可将大肠杆菌中β-半乳糖苷酶的合成抑制高达200倍。它们通过与特定的对称乳糖Oc操纵子变体特异性结合来实现这一点。乳糖阻遏物中的突变定位在识别螺旋的两个不同部分，包括：（i）与乳糖操纵子的碱基对4和5相互作用的残基1和2，以及（ii）识别操纵子碱基对6的残基6。残基1和2的突变可与残基6的突变结合。产生的突变蛋白与在碱基对4、5和6中有三个对称交换的操纵子变体特异性结合。
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本文引用的文献
1
Nucleotide sequence and expression of Escherichia coli trpR, the structural gene for the trp aporepressor.大肠杆菌色氨酸脱辅基阻遏物的结构基因trpR的核苷酸序列及表达
Proc Natl Acad Sci U S A. 1980 Dec;77(12):7117-21. doi: 10.1073/pnas.77.12.7117.
2
Structure of the DNA-binding region of lac repressor inferred from its homology with cro repressor.从乳糖阻遏物与Cro阻遏物的同源性推断出的乳糖阻遏物DNA结合区域的结构。
Proc Natl Acad Sci U S A. 1982 Mar;79(5):1428-32. doi: 10.1073/pnas.79.5.1428.
3
Molecular basis of DNA sequence recognition by the catabolite gene activator protein: detailed inferences from three mutations that alter DNA sequence specificity.分解代谢物基因激活蛋白识别DNA序列的分子基础：来自三个改变DNA序列特异性的突变的详细推断
Proc Natl Acad Sci U S A. 1984 Dec;81(23):7274-8. doi: 10.1073/pnas.81.23.7274.
4
Possible ideal lac operator: Escherichia coli lac operator-like sequences from eukaryotic genomes lack the central G X C pair.可能的理想乳糖操纵子：来自真核生物基因组的大肠杆菌乳糖操纵子样序列缺乏中心G X C对。
Proc Natl Acad Sci U S A. 1984 Mar;81(6):1624-8. doi: 10.1073/pnas.81.6.1624.
5
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6
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Proc Natl Acad Sci U S A. 1982 Apr;79(8):2427-31. doi: 10.1073/pnas.79.8.2427.
7
Cloning and sequence of the crp gene of Escherichia coli K 12.大肠杆菌K12 crp基因的克隆与序列分析
Nucleic Acids Res. 1982 Feb 25;10(4):1363-78. doi: 10.1093/nar/10.4.1363.
8
Protein-DNA recognition.蛋白质-脱氧核糖核酸识别
Annu Rev Biochem. 1984;53:293-321. doi: 10.1146/annurev.bi.53.070184.001453.
9
The molecular basis of DNA-protein recognition inferred from the structure of cro repressor.从cro阻遏蛋白结构推断的DNA-蛋白质识别的分子基础。
Nature. 1982 Aug 19;298(5876):718-23. doi: 10.1038/298718a0.
10
Mutations that alter the DNA sequence specificity of the catabolite gene activator protein of E. coli.改变大肠杆菌分解代谢基因激活蛋白DNA序列特异性的突变。
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