Boch Tanja, Tennert Christian, Vach Kirstin, Al-Ahmad Ali, Hellwig Elmar, Polydorou Olga
Department of Operative Dentistry and Periodontology, University Medical Center Freiburg, Hugstetterstraße 55, 79106, Freiburg i. Br, Germany.
Center for Medical Biometry and Medical Informatics, Institute for Medical Biometry and Statistics, Medical Center-University of Freiburg, Stefan-Meier-Str. 26, 79104, Freiburg, Germany.
Clin Oral Investig. 2016 Sep;20(7):1733-9. doi: 10.1007/s00784-015-1667-1. Epub 2015 Dec 4.
The aim of this study was to evaluate the antimicrobial effect of gaseous ozone compared to conventional methods against Enterococcus faecalis.
One hundred twenty-five teeth were infected by E. faecalis and were incubated for 72 h to form biofilm. Teeth were distributed among five groups. In the first group, ozone was used; in the second group, teeth were rinsed with 20 % ethylenediaminetetraacetic acid (EDTA); in the third group, with 3 % sodium hypochlorite (NaOCl). Group 4 combined 20 % EDTA with ozone. NaOCl and ozone were combined in group 5. After treatment, the samples with paper points were taken, followed by dentin samples taken with K-file, and cultured for 24 h. Then bacterial colonies were counted.
All treatments reduced significantly (p < 0.05) the bacteria. Paper points' samples showed 85.38 % reduction after ozone. The highest reduction was observed in NaOCl group (99.98 %). EDTA reduced bacteria by 80.64 %. Combination of NaOCl and ozone eradicated 99.95 % of the bacteria. Combination of EDTA and ozone reduced E. faecalis up to 91.33 %. The dentin chips showed the following: the highest CFU counts were observed in EDTA group, followed by ozone and NaOCl group. The lowest CFU counts were found in NaOCl-ozone group and EDTA-ozone group.
Ozone reduced E. faecalis, even organised in a biofilm, however, lower than NaOCl. No treatment reduced totally the bacteria.
Used as an adjuvant, ozone can increase the efficacy of conventional rinsing like EDTA and presents an alternative treatment when NaOCl cannot be used e.g. in teeth with a wide-open apical foramen.
本研究旨在评估气态臭氧与传统方法相比对粪肠球菌的抗菌效果。
125颗牙齿被粪肠球菌感染，并培养72小时以形成生物膜。牙齿被分为五组。第一组使用臭氧；第二组用20%乙二胺四乙酸（EDTA）冲洗牙齿；第三组用3%次氯酸钠（NaOCl）冲洗。第四组将20% EDTA与臭氧联合使用。第五组将NaOCl和臭氧联合使用。处理后，用纸尖取样，然后用K锉取牙本质样本，并培养24小时。然后对细菌菌落进行计数。
所有处理均显著减少（p<0.05）细菌数量。纸尖样本在臭氧处理后显示减少了85.38%。次氯酸钠组减少最多（99.98%）。EDTA使细菌减少了80.64%。次氯酸钠和臭氧联合使用根除了99.95%的细菌。EDTA和臭氧联合使用使粪肠球菌减少至91.33%。牙本质碎屑显示如下：EDTA组的菌落形成单位（CFU）计数最高，其次是臭氧组和次氯酸钠组。次氯酸钠 - 臭氧组和EDTA - 臭氧组的CFU计数最低。
臭氧可减少粪肠球菌，即使其形成生物膜，但效果低于次氯酸钠。没有一种处理能完全消除细菌。
作为辅助手段，臭氧可提高像EDTA这样的传统冲洗方法的疗效，并且在不能使用次氯酸钠时（例如根尖孔开放的牙齿）提供了一种替代治疗方法。
