Cunha Gerald R, Baskin Laurence
Department of Urology, Box 0738, University of California, San Francisco, CA 94143, United States.
Department of Urology, Box 0738, University of California, San Francisco, CA 94143, United States.
Differentiation. 2016 Apr-Jun;91(4-5):4-9. doi: 10.1016/j.diff.2015.10.007. Epub 2015 Nov 27.
The sub-renal capsule graft site for in vivo growth and development of developing organs can be used to great advantage in the "rescue" of organ rudiments from "embryonic" or "birth" lethal mutant mice, which permits examination of the full impact of gene knockout in all phases of development from morphogenesis to adult functional differentiation. Another use of the sub-renal capsule graft site is the examination of normal and "chemically perturbed" development of human fetal organs. Tissue recombinants composed of various types of epithelium and mesenchyme, when grafted under the renal capsule undergo normal development and in 3-4 weeks achieve full adult functional cytodifferentiation. The investigator can control many of the developmental parameters of the graft such as endocrine status of the host and treatment of the host with a variety of biologically active agents to assess their effects on development and differentiation.
用于发育中器官体内生长和发育的肾下囊移植位点，在从“胚胎”或“出生”致死突变小鼠中“拯救”器官原基方面具有很大优势，这使得能够在从形态发生到成年功能分化的所有发育阶段检查基因敲除的全面影响。肾下囊移植位点的另一个用途是检查人类胎儿器官的正常和“化学扰动”发育。由各种类型的上皮和间充质组成的组织重组体，移植到肾囊下后会经历正常发育，并在3 - 4周内实现完全的成年功能细胞分化。研究人员可以控制移植的许多发育参数，如宿主的内分泌状态以及用各种生物活性剂对宿主进行处理，以评估它们对发育和分化的影响。
