通过磷光寿命测量对体内细胞内氧张力进行定量分析。
Quantitating intracellular oxygen tension in vivo by phosphorescence lifetime measurement.
作者信息
Hirakawa Yosuke, Yoshihara Toshitada, Kamiya Mako, Mimura Imari, Fujikura Daichi, Masuda Tsuyoshi, Kikuchi Ryohei, Takahashi Ippei, Urano Yasuteru, Tobita Seiji, Nangaku Masaomi
机构信息
Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Graduate School of Science and Technology, Gunma University, 1-5-1 Tenjincho, Kiryu, Gunma 376-8515, Japan.
出版信息
Sci Rep. 2015 Dec 8;5:17838. doi: 10.1038/srep17838.
Hypoxia appears to have an important role in pathological conditions in many organs such as kidney; however, a method to quantify intracellular oxygen tension in vivo has not been well established. In this study, we established an optical method to quantify oxygen tension in mice kidneys using a cationic lipophilic phosphorescence probe, BTPDM1, which has an intracellular oxygen concentration-sensitive phosphorescence lifetime. Since this probe is distributed inside the tubular cells of the mice kidney, we succeeded in detecting acute renal hypoxic conditions and chronic kidney disease. This technique enabled us to estimate intracellular partial pressures of oxygen in vivo by extrapolating the calibration curve generated from cultured tubular cells. Since intracellular oxygen tension is directly related to cellular hypoxic reactions, such as the activation of hypoxia-inducible factors, our method will shed new light on hypoxia research in vivo.
缺氧似乎在包括肾脏在内的许多器官的病理状况中发挥着重要作用；然而，一种在体内定量细胞内氧张力的方法尚未得到很好的确立。在本研究中，我们建立了一种光学方法，使用阳离子亲脂性磷光探针BTPDM1来定量小鼠肾脏中的氧张力，该探针具有对细胞内氧浓度敏感的磷光寿命。由于该探针分布在小鼠肾脏的肾小管细胞内，我们成功检测到了急性肾缺氧状况和慢性肾病。这项技术使我们能够通过外推从培养的肾小管细胞生成的校准曲线来估计体内细胞内的氧分压。由于细胞内氧张力与细胞缺氧反应直接相关，如缺氧诱导因子的激活，我们的方法将为体内缺氧研究提供新的思路。
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