Han Chunli, Ni Yuzhu, Yang Guohao, Yang Jialin, Zou Jie, Peng Huijing, Wang Pengliang
Guangxi Key Laboratory of Marine Environment Change and Disaster in Beibu Gulf, Pinglu Canal and Beibu Gulf Coastal Ecosystem Observation and Research Station of Guangxi, College of Marine Sciences, Beibu Gulf University, Qinzhou, Guangxi, China.
Guangxi Institute of Oceanology, Beihai, Guangxi, China.
PeerJ. 2025 Aug 28;13:e19903. doi: 10.7717/peerj.19903. eCollection 2025.
The marine species , valued for its medicinal and commercial significance, faces critical research gaps due to a lack of molecular markers (notably simple sequence repeats (SSRs)) and insufficient genetic diversity data, hindering genetic studies and evidence-based breeding initiatives.
The software of Misa and Primer3 were adopted to detect SSRs and develop primer pairs, and then some primers of highly polymorphic loci in the genome were used to reveal the genetic diversity of along the Beibu Gulf in China.
From the genome, 277,264 SSRs were detected an d 198,902 primers were designed. Ninety-five of the synthesized 100 primers could amplify across samples, with 93 showing polymorphism. Thirty highly polymorphic primers were chosen to disclose the genetic diversity of 219 materials from Beibu Gulf, China. We detected 370 alleles with high genetic diversity: observed heterozygosity () = 0.905, expected heterozygosity () = 0.833, Shannon's index () = 2.042, and polymorphism information content () = 0.811. The study also revealed varying levels of genetic differentiation and gene flow among different provenances. Structure analysis partitioned all samples into seven distinct genetic clusters.
We identified 277,264 SSRs in and developed primers for 198,902 SSRs. Subsequently, 100 primers were validated for assessing genetic diversity and population differentiation. These will establish a critical foundation for advancing germplasm conservation and targeted breeding strategies in this species.
该海洋物种因其药用和商业价值而备受关注，但由于缺乏分子标记（尤其是简单序列重复序列（SSRs））以及遗传多样性数据不足，面临着关键的研究空白，这阻碍了遗传研究和基于证据的育种计划。
采用Misa和Primer3软件检测SSR并开发引物对，然后使用基因组中一些高度多态性位点的引物来揭示中国北部湾沿岸该物种的遗传多样性。
从基因组中检测到277,264个SSR，并设计了198,902对引物。合成的100对引物中有95对能在样本间扩增，其中93对表现出多态性。选择30对高度多态性引物来揭示来自中国北部湾的219份材料的遗传多样性。我们检测到370个等位基因，具有高遗传多样性：观察杂合度（Ho）=0.905，期望杂合度（He）=0.833，香农指数（I）=2.042，多态信息含量（PIC）=0.811。该研究还揭示了不同种源间不同程度的遗传分化和基因流。结构分析将所有样本划分为七个不同的遗传簇。
我们在该物种中鉴定出277,264个SSR，并为198,902个SSR开发了引物。随后，验证了100对引物用于评估遗传多样性和群体分化。这些将为推进该物种的种质保护和定向育种策略奠定关键基础。
