CSIRO Livestock Industries, Australian Animal Health Laboratory, P.O. Bag 24, Geelong, VIC 3220, Australia.
Dev Comp Immunol. 2012 Mar;36(3):610-8. doi: 10.1016/j.dci.2011.11.001. Epub 2011 Nov 7.
Bats are natural reservoir hosts to a variety of viruses, many of which cause morbidity and mortality in other mammals. Currently there is a paucity of information regarding the nature of the immune response to viral infections in bats, partly due to a lack of appropriate bat specific reagents. IFNγ plays a key role in controlling viral replication and coordinating a response for long term control of viral infection. Here we describe the cloning and expression of IFNγ from the Australian flying fox, Pteropus alecto and the generation of mouse monoclonal and chicken egg yolk antibodies specific to bat IFNγ. Our results demonstrate that P. alecto IFNγ is conserved with IFNγ from other species and is induced in bat splenocytes following stimulation with T cell mitogens. P. alecto IFNγ has antiviral activity on Semliki forest virus in cell lines from P. alecto and the microbat, Tadarida brasiliensis. Additionally recombinant bat IFNγ was able to mitigate Hendra virus infection in P. alecto cells. These results provide the first evidence for an antiviral role for bat IFNγin vitro in addition to the application of important immunological reagents for further studies of bat antiviral immunity.
蝙蝠是多种病毒的天然宿主库，其中许多病毒会导致其他哺乳动物发病和死亡。目前，有关蝙蝠对病毒感染的免疫反应性质的信息很少，部分原因是缺乏适当的蝙蝠特异性试剂。IFNγ 在控制病毒复制和协调长期控制病毒感染的反应中发挥着关键作用。在这里，我们描述了来自澳大利亚飞狐（Pteropus alecto）的 IFNγ 的克隆和表达，并生成了针对蝙蝠 IFNγ 的小鼠单克隆抗体和鸡卵黄抗体。我们的结果表明，P. alecto IFNγ与其他物种的 IFNγ 保守，并在 T 细胞有丝分裂原刺激后诱导蝙蝠脾细胞产生。P. alecto IFNγ对 Semliki 森林病毒在来自 P. alecto 和小蝙蝠 Tadarida brasiliensis 的细胞系中具有抗病毒活性。此外，重组蝙蝠 IFNγ能够减轻 Hendra 病毒在 P. alecto 细胞中的感染。这些结果除了提供了用于进一步研究蝙蝠抗病毒免疫的重要免疫试剂外，还首次证明了蝙蝠 IFNγ 在体外具有抗病毒作用。
