Liu Wenxian, Zhang Zhengshe, Chen Shuangyan, Ma Lichao, Wang Hucheng, Dong Rui, Wang Yanrong, Liu Zhipeng
State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, China.
Key Laboratory of Plant Resources, Institute of Botany, The Chinese Academy of Sciences, Beijing, China.
Plant Cell Rep. 2016 Mar;35(3):561-71. doi: 10.1007/s00299-015-1903-9. Epub 2015 Dec 8.
We studied the genome-wide multiple time-course transcriptome dynamics after saliva deposition in alfalfa and demonstrate that saliva deposition functions as a stress that negatively affects the regrowth of alfalfa. Saliva deposition is one of the key factors influencing plant-herbivore interactions during grazing. Although many studies have focused on the effects of saliva deposition on plant regrowth, no consistent conclusions have been reached. Alfalfa is the most extensively cultivated forage legume, yet most alfalfa cultivars, thus far, are not grazing-tolerant. To better understand the underlying mechanism, we undertook a study to evaluate the global changes in the transcriptome of alfalfa after cow saliva deposition treatment. In this study, cDNA libraries from alfalfa seedlings at 0, 4, 8, and 24 h after cow saliva deposition were constructed and sequenced, resulting in the identification of 53,195 annotated unigenes, from which 4,814 unigenes were significantly differentially expressed. A metabolic pathway enrichment analysis demonstrated that saliva deposition functions as a stress that negatively affects the regrowth of alfalfa by modifying jasmonic acid synthesis, enhancing the susceptibility to pathogens and reducing the expression levels of ribosomal protein genes. In the present study, we demonstrate the potential effects of saliva deposition on alfalfa regrowth at the transcriptome level. These fundamental and important findings could facilitate further investigations into the molecular mechanisms underlying the responses of alfalfa and other related species to herbivore grazing.
我们研究了苜蓿中唾液沉积后的全基因组多时间进程转录组动态变化，并证明唾液沉积作为一种胁迫对苜蓿的再生长产生负面影响。唾液沉积是放牧期间影响植物与食草动物相互作用的关键因素之一。尽管许多研究聚焦于唾液沉积对植物再生长的影响，但尚未达成一致结论。苜蓿是种植最广泛的豆科牧草，然而到目前为止，大多数苜蓿品种不耐放牧。为了更好地理解其潜在机制，我们开展了一项研究来评估奶牛唾液沉积处理后苜蓿转录组的全局变化。在本研究中，构建并测序了奶牛唾液沉积后0、4、8和24小时苜蓿幼苗的cDNA文库，鉴定出53,195个注释的单基因，其中4,814个单基因有显著差异表达。代谢途径富集分析表明，唾液沉积作为一种胁迫，通过改变茉莉酸合成、增强对病原体的易感性以及降低核糖体蛋白基因的表达水平，对苜蓿的再生长产生负面影响。在本研究中，我们在转录组水平上证明了唾液沉积对苜蓿再生长的潜在影响。这些基础且重要的发现有助于进一步研究苜蓿及其他相关物种对食草动物放牧反应的分子机制。
