基于DNA的药用金盏花（菊科）鉴定。

DNA-based identification of Calendula officinalis (Asteraceae).

作者信息

Schmiderer Corinna, Lukas Brigitte, Ruzicka Joana, Novak Johannes

机构信息

University of Veterinary Medicine, Institute of Animal Nutrition and Functional Plant Compounds, Veterinärplatz 1, 1210 Vienna, Austria.

出版信息

Appl Plant Sci. 2015 Nov 3;3(11). doi: 10.3732/apps.1500069. eCollection 2015 Nov.

DOI:10.3732/apps.1500069

PMID:26649268

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4651632/

Abstract

PREMISE OF THE STUDY

For the economically important species Calendula officinalis, a fast identification assay based on high-resolution melting curve analysis was designed. This assay was developed to distinguish C. officinalis from other species of the genus and other Asteraceae genera, and to detect C. officinalis as an adulterant of saffron samples.

METHODS AND RESULTS

For this study, five markers (ITS, rbcL, 5' trnK-matK, psbA-trnH, trnL-trnF) of 10 Calendula species were sequenced and analyzed for species-specific mutations. With the application of two developed primer pairs located in the trnK 5' intron and trnL-trnF, C. officinalis could be distinguished from other species of the genus and all outgroup samples tested. Adulterations of Calendula DNA in saffron could be detected down to 0.01%.

CONCLUSIONS

With the developed assay, C. officinalis can be reliably identified and admixtures of this species as adulterant of saffron can be revealed at low levels.

摘要

研究前提

针对具有重要经济价值的金盏花物种，设计了一种基于高分辨率熔解曲线分析的快速鉴定方法。该方法旨在区分金盏花与该属的其他物种以及其他菊科属的物种，并检测金盏花作为藏红花样品掺假物的情况。

方法与结果

在本研究中，对10种金盏花物种的5个标记（ITS、rbcL、5' trnK - matK、psbA - trnH、trnL - trnF）进行了测序，并分析了物种特异性突变。通过应用位于trnK 5'内含子和trnL - trnF中的两个开发引物对，可以区分金盏花与该属的其他物种以及所有测试的外类群样品。藏红花中低至0.01%的金盏花DNA掺假也能被检测到。