Department of Physical Chemistry of Supramolecular Complexes, Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland.
Department of Physical Chemistry of Supramolecular Complexes, Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland.
Biotechnol Adv. 2016 Jan-Feb;34(1):30-46. doi: 10.1016/j.biotechadv.2015.12.002. Epub 2015 Dec 3.
The present review article focuses on gathering, summarizing, and critically evaluating the results of the last decade on separating and sensing macromolecular compounds and microorganisms with the use of molecularly imprinted polymer (MIP) synthetic receptors. Macromolecules play an important role in biology and are termed that way to contrast them from micromolecules. The former are large and complex molecules with relatively high molecular weights. The article mainly considers chemical sensing of deoxyribonucleic acids (DNAs), proteins and protein fragments as well as sugars and oligosaccharides. Moreover, it briefly discusses fabrication of chemosensors for determination of bacteria and viruses that can ultimately be considered as extremely large macromolecules.
本文综述了过去十年利用分子印迹聚合物（MIP）合成受体分离和检测生物大分子化合物和微生物的研究成果。大分子在生物学中起着重要的作用，它们被称为大分子，以与小分子相对比。前者是具有相对较高分子量的大型复杂分子。本文主要考虑脱氧核糖核酸（DNA）、蛋白质和蛋白质片段以及糖和寡糖的化学传感。此外，还简要讨论了用于测定细菌和病毒的化学传感器的制备，这些细菌和病毒最终可以被认为是非常大的大分子。
