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大肠杆菌谷氨酰胺基因（glnG）中的突变导致氮调节因子I的活性增加。

Mutations in the glnG gene of Escherichia coli that result in increased activity of nitrogen regulator I.

作者信息

Weglenski P, Ninfa A J, Ueno-Nishio S, Magasanik B

机构信息

Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.

出版信息

J Bacteriol. 1989 Aug;171(8):4479-85. doi: 10.1128/jb.171.8.4479-4485.1989.

DOI:10.1128/jb.171.8.4479-4485.1989
PMID:2666403
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC210228/
Abstract

Mutations in the glnG gene of Escherichia coli that result in increased activity of nitrogen regulator I (NRI), the product of glnG, were obtained by two different selection procedures. The mutant proteins were purified and characterized. The concentrations of mutant proteins needed to activate transcription at the glnAp2 promoter were three to four times lower than that of the wild-type NRI. The rate of phosphorylation of these proteins and the stability of mutant NRI phosphate were found to be similar to those of the wild-type NRI. In one of the mutants, the site of the mutation was localized in the DNA region specifying the central domain of NRI.

摘要

通过两种不同的筛选程序获得了大肠杆菌谷氨酰胺合成酶基因（glnG）中的突变，这些突变导致谷氨酰胺合成酶基因产物氮调节蛋白I（NRI）的活性增加。对突变蛋白进行了纯化和特性分析。激活glnAp2启动子转录所需的突变蛋白浓度比野生型NRI低三到四倍。发现这些蛋白的磷酸化速率和突变型NRI磷酸盐的稳定性与野生型NRI相似。在其中一个突变体中，突变位点位于指定NRI中央结构域的DNA区域。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a84b/210228/ab7ed61890b0/jbacter00174-0391-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a84b/210228/e4bda80bf16d/jbacter00174-0389-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a84b/210228/d4d0cb3d8f40/jbacter00174-0390-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a84b/210228/ab7ed61890b0/jbacter00174-0391-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a84b/210228/e4bda80bf16d/jbacter00174-0389-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a84b/210228/d4d0cb3d8f40/jbacter00174-0390-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a84b/210228/ab7ed61890b0/jbacter00174-0391-a.jpg

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本文引用的文献

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Isolation of the nitrogen assimilation regulator NR(I), the product of the glnG gene of Escherichia coli.分离出氮同化调节剂 NR(I)，它是大肠杆菌 glnG 基因的产物。
Proc Natl Acad Sci U S A. 1983 Sep;80(18):5554-8. doi: 10.1073/pnas.80.18.5554.
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Regulation of nitrogen fixation genes.固氮基因的调控
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The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.pUC质粒，一种源自M13mp7的用于插入诱变和使用合成通用引物进行测序的系统。
通过荧光各向异性和荧光相关光谱研究NtrC的DNA结合与寡聚化。
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CRP interacts with promoter-bound sigma54 RNA polymerase and blocks transcriptional activation of the dctA promoter.CRP与结合在启动子上的σ54 RNA聚合酶相互作用，并阻断dctA启动子的转录激活。
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The glnB region of the Escherichia coli chromosome.大肠杆菌染色体的glnB区域。
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Active contribution of two domains to cooperative DNA binding of the enhancer-binding protein nitrogen regulator I (NtrC) of Escherichia coli: stimulation by phosphorylation and the binding of ATP.大肠杆菌增强子结合蛋白氮调节因子I（NtrC）的两个结构域对协同DNA结合的积极贡献：磷酸化刺激和ATP结合
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A common switch in activation of the response regulators NtrC and PhoB: phosphorylation induces dimerization of the receiver modules.响应调节因子NtrC和PhoB激活过程中的一个常见转换：磷酸化诱导接收模块二聚化。
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Functions of the gene products of Escherichia coli.大肠杆菌基因产物的功能。
Microbiol Rev. 1993 Dec;57(4):862-952. doi: 10.1128/mr.57.4.862-952.1993.
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Protein phosphorylation and regulation of adaptive responses in bacteria.细菌中的蛋白质磷酸化与适应性反应的调控
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Identification and regulation of the glnL operator-promoter of the complex glnALG operon of Escherichia coli.大肠杆菌复杂的谷氨酰胺合成酶基因操纵子（glnALG operon）中谷氨酰胺合成酶基因L（glnL）操纵子-启动子的鉴定与调控
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The product of glnL is not essential for regulation of bacterial nitrogen assimilation.谷氨酰胺合成酶基因（glnL）的产物对于细菌氮同化的调节并非必不可少。
J Bacteriol. 1983 Apr;154(1):516-9. doi: 10.1128/jb.154.1.516-519.1983.
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Characterization of a gene, glnL, the product of which is involved in the regulation of nitrogen utilization in Escherichia coli.一个基因（glnL）的特性，其产物参与大肠杆菌氮利用的调控。
J Bacteriol. 1982 Apr;150(1):214-20. doi: 10.1128/jb.150.1.214-220.1982.
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Complex glnA-glnL-glnG operon of Escherichia coli.大肠杆菌的复杂谷氨酰胺合成酶基因-谷氨酰胺基因-谷氨酰胺调节基因操纵子
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Physical and genetic characterization of the glnA--glnG region of the Escherichia coli chromosome.大肠杆菌染色体谷氨酰胺合成酶基因（glnA）-谷氨酰胺合成酶基因激活蛋白基因（glnG）区域的物理和遗传特征分析
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Localized mutagenesis of any specific small region of the bacterial chromosome.细菌染色体任何特定小区域的定位诱变。
Proc Natl Acad Sci U S A. 1971 Dec;68(12):3158-62. doi: 10.1073/pnas.68.12.3158.
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Deduced products of C4-dicarboxylate transport regulatory genes of Rhizobium leguminosarum are homologous to nitrogen regulatory gene products.豌豆根瘤菌C4-二羧酸转运调控基因的推导产物与氮调控基因产物同源。
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