逆转录聚合酶链反应在裂谷热早期诊断中的应用。
Use of reverse transcriptase PCR in early diagnosis of Rift Valley fever.
作者信息
Sall A A, Macondo E A, Sène O K, Diagne M, Sylla R, Mondo M, Girault L, Marrama L, Spiegel A, Diallo M, Bouloy M, Mathiot C
机构信息
Institut Pasteur de Dakar, Dakar, Senegal.
出版信息
Clin Diagn Lab Immunol. 2002 May;9(3):713-5. doi: 10.1128/cdli.9.3.713-715.2002.
Abstract
Reverse transcriptase PCR (RT-PCR) for diagnosis of Rift Valley fever (RVF) was evaluated by using 293 human and animal sera sampled during an RVF outbreak in Mauritania in 1998. Results of the RT-PCR diagnostic method were compared with those of virus isolation (VI) and detection of immunoglobulin M (IgM) antibodies. Our results showed that RT-PCR is a specific, sensitive tool for RVF diagnosis in the early phase of the disease and that its results do not differ significantly from those obtained by VI. Moreover, the combined results of RT-PCR and IgM antibody detection were in 100% concordance with the results of VI.
摘要
采用1998年毛里塔尼亚裂谷热疫情期间采集的293份人和动物血清，对用于诊断裂谷热（RVF）的逆转录酶聚合酶链反应（RT-PCR）进行了评估。将RT-PCR诊断方法的结果与病毒分离（VI）和免疫球蛋白M（IgM）抗体检测结果进行了比较。我们的结果表明，RT-PCR是疾病早期诊断裂谷热的一种特异性、灵敏的工具，其结果与通过病毒分离获得的结果没有显著差异。此外，RT-PCR和IgM抗体检测的综合结果与病毒分离结果的一致性为100%。
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