人肠上皮细胞/结肠类器官和肠道类器官在功能上概括了正常肠道生理学和病理生理学。
Human Enteroids/Colonoids and Intestinal Organoids Functionally Recapitulate Normal Intestinal Physiology and Pathophysiology.
作者信息
Zachos Nicholas C, Kovbasnjuk Olga, Foulke-Abel Jennifer, In Julie, Blutt Sarah E, de Jonge Hugo R, Estes Mary K, Donowitz Mark
机构信息
From the Division of Gastroenterology and Hepatology, Department of Medicine, and.
the Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas 77030, and.
出版信息
J Biol Chem. 2016 Feb 19;291(8):3759-66. doi: 10.1074/jbc.R114.635995. Epub 2015 Dec 16.
Identification of Lgr5 as the intestinal stem cell marker as well as the growth factors necessary to replicate adult intestinal stem cell division has led to the establishment of the methods to generate "indefinite" ex vivo primary intestinal epithelial cultures, termed "mini-intestines." Primary cultures developed from isolated intestinal crypts or stem cells (termed enteroids/colonoids) and from inducible pluripotent stem cells (termed intestinal organoids) are being applied to study human intestinal physiology and pathophysiology with great expectations for translational applications, including regenerative medicine. Here we discuss the physiologic properties of these cultures, their current use in understanding diarrhea-causing host-pathogen interactions, and potential future applications.
Lgr5被鉴定为肠道干细胞标志物，以及复制成体肠道干细胞分裂所需的生长因子，这导致了产生“无限”体外原代肠上皮培养物（称为“迷你肠”）方法的建立。从分离的肠隐窝或干细胞（称为肠类器官/结肠类器官）以及诱导多能干细胞（称为肠道类器官）发育而来的原代培养物正被用于研究人类肠道生理学和病理生理学，人们对其在包括再生医学在内的转化应用寄予厚望。在此，我们讨论这些培养物的生理特性、它们目前在理解腹泻引起的宿主 - 病原体相互作用中的应用以及潜在的未来应用。
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