Liang Wulong, He Lei, Ning Pengbo, Lin Jihui, Li Helin, Lin Zhi, Kang Kai, Zhang Yanming
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
Animal Disease and Public Security Academician Workstation of Henan Province, The Key Lab of Animal Disease and Public Security, Henan University of Science and Technology, Luoyang 471003, PR China.
Res Vet Sci. 2015 Dec;103:28-33. doi: 10.1016/j.rvsc.2015.09.009. Epub 2015 Sep 12.
Transmissible gastroenteritis virus (TGEV) causes transmissible gastroenteritis (TGE), especially in newborn piglets, which severely threatens the worldwide pig industry. In this study, (+)-catechin was evaluated for its antiviral effect against TGEV in vitro. Viability assays revealed that (+)-catechin treatment exerted a dose-dependent rescue effect in TGEV-infected ST cells, and this result was only obtained with the post-treatment application of (+)-catechin. The viral yields in (+)-catechin-treated cultures were reduced by almost three log10 units. Quantitative real-time PCR analysis of the TGEV genome revealed that TGEV RNA replication was restricted after (+)-catechin treatment. Intracellular reactive oxygen species (ROS) detection showed that (+)-catechin alleviated ROS conditions induced by TGEV infection. Our results showed that (+)-catechin exerts an inhibitory effect on TGEV proliferation in vitro and is involved its antioxidation.
传染性胃肠炎病毒（TGEV）可引发传染性胃肠炎（TGE），尤其是对新生仔猪，这严重威胁着全球养猪业。在本研究中，对（+）-儿茶素在体外抗TGEV的抗病毒作用进行了评估。活力测定表明，（+）-儿茶素处理对TGEV感染的ST细胞具有剂量依赖性的挽救作用，且该结果仅在（+）-儿茶素后处理时获得。（+）-儿茶素处理的培养物中的病毒产量降低了近三个对数10单位。对TGEV基因组的定量实时PCR分析表明，（+）-儿茶素处理后TGEV RNA复制受到限制。细胞内活性氧（ROS）检测表明，（+）-儿茶素减轻了TGEV感染诱导的ROS状况。我们的结果表明，（+）-儿茶素在体外对TGEV增殖具有抑制作用，并参与其抗氧化作用。
