Mossman B T, Marsh J P
Department of Pathology, University of Vermont, Burlington 05405.
Environ Health Perspect. 1989 May;81:91-4. doi: 10.1289/ehp.898191.
Asbestos is an important occupational and environmental toxicant that affects several cell types in the respiratory tract. In an effort to understand how asbestos causes cell injury and/or altered proliferation and differentiation of cells, this laboratory has focused on reactive oxygen species as mediators of asbestos-induced biological effects. A compendium of experimental results reported by this laboratory and others supports this hypothesis. For example, scavengers of reactive oxygen metabolites and iron chelators (i.e., desferroxamine) prevent cytotoxicity after addition of asbestos to a variety of cell lines and macrophages in vitro. DNA strand breakage associated with toxicity of crocidolite asbestos in C3H10T 1/2 cells also is ameliorated with use of desferroxamine. All types of asbestos cause lipid peroxidation in mammalian cells and artificial membranes, a phenomenon that can be prevented by removal of catalytic iron. Last, asbestos causes generation of active oxygen species after interaction with leukocytes or by reduction of oxygen on the surface of the fibers.
石棉是一种重要的职业和环境毒物，会影响呼吸道中的多种细胞类型。为了了解石棉如何导致细胞损伤和/或细胞增殖及分化改变，本实验室将重点放在活性氧作为石棉诱导生物学效应的介质上。本实验室及其他机构报告的一系列实验结果支持这一假说。例如，活性氧代谢产物清除剂和铁螯合剂（即去铁胺）可在体外将石棉添加到多种细胞系和巨噬细胞后预防细胞毒性。使用去铁胺也可改善与青石棉在C3H10T 1/2细胞中的毒性相关的DNA链断裂。所有类型的石棉都会在哺乳动物细胞和人工膜中引起脂质过氧化，这一现象可通过去除催化铁来预防。最后，石棉在与白细胞相互作用后或通过纤维表面的氧还原作用导致活性氧的产生。
