Gupta Hemant, Tiwari Tanushree, Patel Maulik, Mehta Aditya, Ghosh Arpita
Xcelris Labs Ltd., Ahmedabad, Gujarat, India.
Genom Data. 2015 Sep 9;6:139-44. doi: 10.1016/j.gdata.2015.09.005. eCollection 2015 Dec.
MicroRNAs are a newly discovered class of non-protein small RNAs with 22-24 nucleotides. They play multiple roles in biological processes including development, cell proliferation, apoptosis, stress responses and many other cell functions. In this research, several approaches were combined to make a computational prediction of potential miRNAs and their targets in Helianthus annuus (H. annuus). The already available information of the plant miRNAs present in miRBase v21 was used against expressed sequence tags (ESTs). A total of three miRNAs were detected from which one potential novel miRNA was identified following a range of strict filtering criteria. The target prediction was carried out for these three miRNAs having various targets. These targets were functionally annotated and GO terms were assigned. To study the conserved nature of the miRNAs, predicted phylogenetic analysis was carried out. These findings will significantly provide the broader picture for understanding the functions in H. annuus.
微小RNA是一类新发现的非蛋白质小RNA，含有22 - 24个核苷酸。它们在包括发育、细胞增殖、凋亡、应激反应和许多其他细胞功能在内的生物过程中发挥多种作用。在本研究中，结合了几种方法对向日葵（Helianthus annuus）中潜在的微小RNA及其靶标进行计算预测。利用miRBase v21中已有的植物微小RNA信息与表达序列标签（ESTs）进行比对。共检测到三个微小RNA，经过一系列严格的筛选标准后，鉴定出一个潜在的新微小RNA。对这三个具有不同靶标的微小RNA进行了靶标预测。对这些靶标进行了功能注释并赋予了基因本体（GO）术语。为了研究微小RNA的保守性质，进行了预测的系统发育分析。这些发现将为理解向日葵中的功能提供更广阔的图景。
