从铁皮石斛EST数据中挖掘微小RNA

Krishnatreya Debasish B, Baruah Pooja Moni, Dowarah Bhaskar, Bordoloi Kuntala Sarma, Agarwal Heena, Agarwala Niraj

Department of Botany, Gauhati University, Guwahati, Assam, India - 7810014.

Bioinformation. 2020 Mar 31;16(3):245-255. doi: 10.6026/97320630016245. eCollection 2020.

Dendrobium nobile is an orchid species highly popular for its therapeutic properties and is often used as a medicinal herb. Documenting miRNA-target associations in D. nobile is an important step to facilitate functional genomics studies in this species. Therefore, it is of interest to identify miRNA sequences from EST data available in public databases using known techniques and tools. We report 14 potential miRNAs from three ESTs of D. nobile. They belong to 3 miRNA families (miR390, miR528 and miR414) linking to transcription factor regulation, signal transduction, DNA and protein binding, and various cellular processes covering 34 different metabolic networks in KEGG. These results help in the understanding of miRNA-mRNAs functional networks in Dendrobium nobile.

金钗石斛是一种因其治疗特性而广受欢迎的兰花物种，常被用作草药。记录金钗石斛中的miRNA-靶标关联是促进该物种功能基因组学研究的重要一步。因此，利用已知技术和工具从公共数据库中可用的EST数据中识别miRNA序列很有意义。我们从金钗石斛的三个EST中报告了14个潜在的miRNA。它们属于3个miRNA家族（miR390、miR528和miR414），与转录因子调控、信号转导、DNA和蛋白质结合以及KEGG中涵盖34个不同代谢网络的各种细胞过程相关。这些结果有助于理解金钗石斛中miRNA-mRNA的功能网络。