使用紧凑型可见激光二极管激发对浅表微血管进行无标记光学分辨率光声显微镜成像。
Label-free optical-resolution photoacoustic microscopy of superficial microvasculature using a compact visible laser diode excitation.
作者信息
Zeng Lvming, Piao Zhonglie, Huang Shenghai, Jia Wangcun, Chen Zhongping
出版信息
Opt Express. 2015 Nov 30;23(24):31026-33. doi: 10.1364/OE.23.031026.
Abstract
We have developed laser-diode-based optical-resolution photoacoustic microscopy (LD-OR-PAM) of superficial microvasculature which has the desirable properties of being compact, low-cost, and label-free. A 300-mW visible pulsed laser diode was operated at a 405 ± 5 nm wavelength with a pulse energy as low as 52 nJ. By using a 3.6 MHz ultrasound transducer, the system was tested on carbon fibers with a lateral resolution of 0.95 µm and an SNR of 38 dB. The subcutaneous microvasculature on a mouse back was imaged without an exogenous contrast agent which demonstrates the potential of the proposed prototype for skin chromophores. Our eventual goal is to offer a practical and affordable multi-wavelength functional LD-OR-PAM instrument suitable for clinical applications.
摘要
我们已经开发出基于激光二极管的浅表微血管光学分辨率光声显微镜（LD-OR-PAM），它具有紧凑、低成本和无需标记等理想特性。一个300毫瓦的可见脉冲激光二极管在405±5纳米波长下工作，脉冲能量低至52纳焦。通过使用一个3.6兆赫的超声换能器，该系统在碳纤维上进行了测试，横向分辨率为0.95微米，信噪比为38分贝。在没有外源性造影剂的情况下对小鼠背部的皮下微血管进行了成像，这证明了所提出的原型用于皮肤发色团的潜力。我们的最终目标是提供一种适用于临床应用的实用且经济实惠的多波长功能性LD-OR-PAM仪器。
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