结核分枝杆菌DNA增加人单核细胞中维生素D受体mRNA表达以及一氧化氮和杀菌肽的产生。
Mycobacterium tuberculosis DNA Increases Vitamin D Receptor mRNA Expression and the Production of Nitric Oxide and Cathelicidin in Human Monocytes.
作者信息
Siswanto Siswanto, Zuhriyah Lilik, Handono Kusworini, Fitri Loeki Enggar, Prawiro Sumarno Reto
机构信息
Department of Public Health, Faculty of Medicine, University of Brawijaya, Jalan Veteran, Malang, Indonesia.
Department of Clinical Pathology, Faculty of Medicine, University of Brawijaya, Jalan Veteran Malang, Indonesia.
出版信息
Malays J Med Sci. 2015 May-Jun;22(3):18-24.
BACKGROUND
The innate immune response to tuberculosis infection may involve the increased production of nitric oxide and cathelicidin due to the up-regulated expression of the vitamin D receptor (VDR), though this proposed mechanism remains controversial. The aim of this study was to determine how the exposure of human monocytes to Mycobacterium tuberculosis (M. tuberculosis) DNA affects the production of nitric oxide and cathelicidin, as well as the expression of VDR.
METHODS
This study was performed using monocytes obtained from healthy donors. After 24 h incubation, monocytes were stimulated with M. tuberculosis DNA for 18 h to determine the expression of VDR mRNA and the production of nitric oxide and cathelicidin versus non-stimulated cells (the control group).
RESULTS
The expression of VDR mRNA was higher in the monocytes exposed to M. tuberculosis DNA compared to the control group (P = 0.020). Monocytes exposed to M. tuberculosis DNA also showed significantly increased production of nitric oxide and cathelicidin compared to the control group (P = 0.0001; P = 0.028).
CONCLUSION
The stimulation of human monocytes with M. tuberculosis DNA increases the expression of the VDR mRNA and the production of nitric oxide and cathelicidin.
背景
对结核感染的先天性免疫反应可能涉及由于维生素D受体（VDR）表达上调而导致一氧化氮和cathelicidin的产生增加，尽管这一提出的机制仍存在争议。本研究的目的是确定人类单核细胞暴露于结核分枝杆菌（M. tuberculosis）DNA如何影响一氧化氮和cathelicidin的产生以及VDR的表达。
方法
本研究使用从健康供体获得的单核细胞进行。孵育24小时后，用结核分枝杆菌DNA刺激单核细胞18小时，以确定VDR mRNA的表达以及与未刺激细胞（对照组）相比一氧化氮和cathelicidin的产生。
结果
与对照组相比，暴露于结核分枝杆菌DNA的单核细胞中VDR mRNA的表达更高（P = 0.020）。与对照组相比，暴露于结核分枝杆菌DNA的单核细胞中一氧化氮和cathelicidin的产生也显著增加（P = 0.0001；P = 0.028）。
结论
用结核分枝杆菌DNA刺激人类单核细胞可增加VDR mRNA的表达以及一氧化氮和cathelicidin的产生。
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