Altmann M, Trachsel H
Institut für Biochemie und Molekularbiologie Universität Bern, Switzerland.
Nucleic Acids Res. 1989 Aug 11;17(15):5923-31. doi: 10.1093/nar/17.15.5923.
The mutation in the S. cerevisiae cell cycle division mutant cdc33 consists of a single G to A transition in the open reading frame encoding translation initiation factor 4E (eIF-4E). This leads to the substitution of glycine 113 by aspartic acid close to tryptophane 115 in the protein. This mutation reduces cap binding activity of eIF-4E as measured by binding of eIF-4E to m7GDP agarose columns and slows down overall protein synthesis at the non-permissive temperature. Comparison of the cdc33 mutation with other mutations affecting eIF-4E function supports the view that tryptophane residues and their flanking regions are involved in cap binding activity of eIF-4E.
酿酒酵母细胞周期分裂突变体cdc33中的突变，是由编码翻译起始因子4E（eIF - 4E）的开放阅读框中单个G到A的转换组成。这导致蛋白质中靠近色氨酸115的甘氨酸113被天冬氨酸取代。通过eIF - 4E与m7GDP琼脂糖柱的结合测定，这种突变降低了eIF - 4E的帽结合活性，并在非允许温度下减缓了整体蛋白质合成。将cdc33突变与影响eIF - 4E功能的其他突变进行比较，支持了色氨酸残基及其侧翼区域参与eIF - 4E帽结合活性的观点。
