Saito Akemi, Takatori Minako, Takatori Kousuke, Taniguchi Masami
Clinical Research Center for Allergy and Rheumatology, National Sagamihara Hospital.
Arerugi. 2015 Dec;64(10):1313-22. doi: 10.15036/arerugi.64.1313.
Airborne fungi have been surveyed every week during the 20 years from 1993 to 2013 in Sagamihara. This is a follow up of 1983 to 1992 survey at the same sampling station, using the same methods.
We analyzed the transition of airborne fungi of 20-years.
Using a settle plate method, air sampling was performed by exposing 90 mm settle plates containing potato dextrose agar to the air for 10 min. The plates were incubated at 25℃ for 7-14 days. The fungi were identified and counted.
The average of number total fungi colonies during 20 years were 507 colony forming units (CFU)/5 plate every year. The most common fungi found, except for yeasts and sterile mycelium, were Cladosporium, Alternaria, Penicillium, Ulocadium, Fusarium, Arthrinium, Epicoccum, Aureobasidium, Curvularia, Nigrospora and Aspergillus. A significant correlation was observed between the number total fungi clonies and number of Cladosporium colonies. Although Alternaria, Arthrinium, Epicoccum and Curvularia were significantly reduced as compared 10 years from 1983 to 1992, Cladosporium and Penicillium were comparable.
Cladosporium was most predominant fungi during the 20 years. This study is very valuable in that the transition of airborne fungi revealed by long-term measurement.
1993年至2013年的20年间，每周都对相模原市的空气传播真菌进行调查。这是对1983年至1992年在同一采样站使用相同方法进行的调查的后续研究。
分析20年间空气传播真菌的变化情况。
采用沉降平板法，将含有马铃薯葡萄糖琼脂的90毫米沉降平板暴露于空气中10分钟进行空气采样。平板在25℃下培养7 - 14天。对真菌进行鉴定和计数。
20年间真菌菌落总数的平均值为每年507个菌落形成单位（CFU）/5个平板。除酵母和无菌菌丝体外，最常见的真菌是枝孢属、链格孢属、青霉属、弯孢属、镰刀菌属、节菱孢属、附球菌属、黑曲霉属、弯孢霉属、黑孢霉属和曲霉属。真菌菌落总数与枝孢属菌落数之间存在显著相关性。与1983年至1992年的10年相比，链格孢属、节菱孢属、附球菌属和弯孢霉属显著减少，但枝孢属和青霉属数量相当。
枝孢属是20年间最主要的真菌。这项研究非常有价值，因为长期测量揭示了空气传播真菌的变化情况。
