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Phys Chem Chem Phys. 2015 Oct 7;17(37):23724-31. doi: 10.1039/c5cp03523f. Epub 2015 Jul 16.
2
In vitro selection of a peptide aptamer that changes fluorescence in response to verotoxin.体外筛选一种能响应志贺毒素而改变荧光的肽适配体。
Biotechnol Lett. 2015 Mar;37(3):619-25. doi: 10.1007/s10529-014-1719-7. Epub 2014 Nov 6.
3
Construction of a crown ether-like supramolecular library by conjugation of genetically-encoded peptide linkers displayed on bacteriophage T7.通过连接展示在噬菌体T7上的基因编码肽接头构建类冠醚超分子文库。
Chem Commun (Camb). 2014 Apr 18;50(30):3921-3. doi: 10.1039/c4cc00811a. Epub 2014 Mar 11.
4
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Chem Commun (Camb). 2014 Mar 18;50(22):2962-4. doi: 10.1039/c3cc47624c. Epub 2013 Dec 9.
5
Self-assembled DNA hydrogel as switchable material for aptamer-based fluorescent detection of protein.自组装 DNA 水凝胶作为基于适体的荧光检测蛋白质的开关材料。
Anal Chem. 2013 Nov 19;85(22):11077-82. doi: 10.1021/ac4027725. Epub 2013 Nov 5.
6
Gp10 based-thioetherification (10BASE(d)-T) on a displaying library peptide of bacteriophage T7.基于噬菌体T7展示文库肽的Gp10硫醚化反应（10BASE(d)-T）。
Mol Biosyst. 2013 Dec;9(12):2988-91. doi: 10.1039/c3mb70379g. Epub 2013 Sep 27.
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通过10BASE(d)-T获得的用于蛋白质特异性指示的变色和强度增强荧光探针的选择。

Selection of Color-Changing and Intensity-Increasing Fluorogenic Probe as Protein-Specific Indicator Obtained via the 10BASE(d)-T.

作者信息

Taki Masumi, Inoue Hiroaki, Mochizuki Kazuto, Yang Jay, Ito Yuji

机构信息

Department of Engineering Science, Bioscience and Technology Program, The Graduate School of Informatics and Engineering, The University of Electro-Communications (UEC) , 1-5-1 Chofugaoka, Chofu, Tokyo 182-8585, Japan.

Department of Anesthesiology, University of Wisconsin, School of Medicine and Public Health , Madison, Wisconsin 53706 United States.

出版信息

Anal Chem. 2016 Jan 19;88(2):1096-9. doi: 10.1021/acs.analchem.5b04687. Epub 2016 Jan 6.

DOI:10.1021/acs.analchem.5b04687
PMID:26727351
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5077683/
Abstract

To obtain a molecular probe for specific protein detection, we have synthesized fluorogenic probe library of vast diversity on bacteriophage T7 via the gp10 based-thioetherificaion (10BASE(d)-T). A remarkable color-changing and turning-on probe was selected from the library, and its physicochemical properties upon target-specific binding were obtained. Combination analyses of fluorescence emission titration, isothermal titration calorimetry (ITC), and quantitative saturation-transfer difference (STD) NMR measurements, followed by in silico docking simulation, rationalized the most plausible geometry of the ligand-protein interaction.

摘要

为了获得用于特异性蛋白质检测的分子探针，我们通过基于gp10的硫醚化反应（10BASE(d)-T）在噬菌体T7上合成了具有广泛多样性的荧光探针文库。从文库中筛选出了一种显著变色且开启型的探针，并获得了其在特异性结合靶点时的物理化学性质。通过荧光发射滴定、等温滴定量热法（ITC）和定量饱和转移差（STD）核磁共振测量的组合分析，随后进行计算机对接模拟，阐明了配体 - 蛋白质相互作用最合理的几何结构。