Zhu Xiaohua, Zhao Tingbi, Nie Zhou, Miao Zhuang, Liu Yang, Yao Shouzhuo
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, People's Republic of China.
Department of Chemistry, Beijing Key Laboratory for Analytical Methods and Instrumentation, Key Lab of Bioorganic Phosphorus Chemistry and Chemical Biology of Ministry of Education, Tsinghua University, Beijing 100084, China.
Nanoscale. 2016 Jan 28;8(4):2205-11. doi: 10.1039/c5nr07826a.
In this work, nitrogen-doped carbon nanoparticle (N-CNP) modulated turn-on fluorescent probes were developed for rapid and selective detection of histidine. The as synthesized N-CNPs exhibited high fluorescence quantum yield and excellent biocompatibility. The fluorescence of N-CNPs can be quenched selectively by Cu(II) ions with high efficiency, and restored by the addition of histidine owing to the competitive binding of Cu(II) ions and histidine that removes Cu(II) ions from the surface of the N-CNPs. Under the optimal conditions, a linear relationship between the increased fluorescence intensity of N-CNP/Cu(II) ion conjugates and the concentration of histidine was established in the range from 0.5 to 60 μM. The detection limit was as low as 150 nM (signal-to-noise ratio of 3). In addition, the as-prepared N-CNP/Cu(II) ion nanoprobes showed excellent biocompatibility and were applied for a histidine imaging assay in living cells, which presented great potential in the bio-labeling assay and clinical diagnostic applications.
在本研究中，开发了氮掺杂碳纳米颗粒（N-CNP）调制的开启型荧光探针，用于快速、选择性地检测组氨酸。合成的N-CNPs具有高荧光量子产率和优异的生物相容性。N-CNPs的荧光可被Cu(II)离子高效选择性猝灭，加入组氨酸后荧光恢复，这是由于Cu(II)离子与组氨酸的竞争性结合将Cu(II)离子从N-CNPs表面去除。在最佳条件下，N-CNP/Cu(II)离子共轭物荧光强度的增加与组氨酸浓度在0.5至60μM范围内呈线性关系。检测限低至150 nM（信噪比为3）。此外，所制备的N-CNP/Cu(II)离子纳米探针表现出优异的生物相容性，并应用于活细胞中的组氨酸成像分析，在生物标记分析和临床诊断应用中具有巨大潜力。
