State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, People's Republic of China.
School of Chemistry and Chemical Engineering, Hunan Institute of Engineering, Xiangtan, 411104, People's Republic of China.
Mikrochim Acta. 2018 Nov 21;185(12):557. doi: 10.1007/s00604-018-3080-3.
A molecularly imprinted photonic hydrogel (MIPH) is described for the optical determination of L-histidine (L-His). The inverse opal structure of MIPH was obtained by placing silica particles (230 nm) in molecularly imprinted polymer on a glass slide. After being fully etched by hydrofluoric acid, this inverse opal structure brings about a high specific surface and plentiful binding sites for L-His. If L-His is absorbed by the modified MIPH, its average effective refraction coefficient is increased. This causes the Bragg diffraction peak to be red-shifted by about 34 nm as the concentration of L-His increases from 0 to 100 nM. Much smaller diffraction peak shifts are obtained for other amino acids. The detection limit of this method is 10 pM. The response time towards L-His is as short as 60 s. In addition, the sensor can be recovered by treatment with 0.1 M acetic acid/methanol. It was applied to the determination of L-His in drinks sample. Graphical abstract After absorbing L-histidine, the average effective refractive index of this molecularly imprinted photonic hydrogel (MIPH) is increased, and the Bragg diffraction peak is shifted. The shift of the diffraction peak can be used for the detection of L-His.
一种用于光学测定 L-组氨酸（L-His）的分子印迹光子凝胶（MIPH）被描述。通过将二氧化硅颗粒（230nm）放置在玻璃载玻片上的分子印迹聚合物中，获得 MIPH 的反蛋白石结构。完全用氢氟酸蚀刻后，这种反蛋白石结构带来了高比表面积和丰富的 L-His 结合位点。如果 L-His 被修饰的 MIPH 吸收，其平均有效折射系数会增加。这导致布拉格衍射峰随着 L-His 浓度从 0 增加到 100nM 而红移约 34nm。对于其他氨基酸，得到的衍射峰位移要小得多。该方法的检测限为 10pM。对 L-His 的响应时间短至 60s。此外，传感器可以用 0.1M 乙酸/甲醇处理进行回收。它被应用于饮料样品中 L-His 的测定。
