Duyck Joke, Vandamme Katleen, Krausch-Hofmann Stefanie, Boon Lies, De Keersmaecker Katrien, Jalon Eline, Teughels Wim
BIOMAT & Prosthetics section - Department of Oral Health Sciences, KU Leuven & University Hospitals Leuven, Leuven, Belgium.
Periodontology section - Department of Oral Health Sciences, KU Leuven & University Hospitals Leuven, Leuven, Belgium.
PLoS One. 2016 Jan 5;11(1):e0145837. doi: 10.1371/journal.pone.0145837. eCollection 2016.
Appropriate oral hygiene is required to maintain oral health in denture wearers. This study aims to compare the role of denture cleaning methods in combination with overnight storage conditions on biofilm mass and composition on acrylic removable dentures.
In a cross-over randomized controlled trial in 13 older people, 4 conditions with 2 different mechanical cleaning methods and 2 overnight storage conditions were considered: (i) brushing and immersion in water without a cleansing tablet, (ii) brushing and immersion in water with a cleansing tablet, (iii) ultrasonic cleaning and immersion in water without a cleansing tablet, and (iv) ultrasonic cleaning and immersion in water with a cleansing tablet. Each test condition was performed for 5 consecutive days, preceded by a 2-days wash-out period. Biofilm samples were taken at baseline (control) and at the end of each test period from a standardized region. Total and individual levels of selected oral bacteria (n = 20), and of Candida albicans were identified using the Polymerase Chain Reaction (PCR) technique. Denture biofilm coverage was scored using an analogue denture plaque score. Paired t-tests and Wilcoxon-signed rank tests were used to compare the test conditions. The level of significance was set at α< 5%.
Overnight denture storage in water with a cleansing tablet significantly reduced the total bacterial count (p<0.01). The difference in total bacterial level between the two mechanical cleaning methods was not statistically significant. No significant effect was observed on the amount of Candida albicans nor on the analogue plaque scores.
The use of cleansing tablets during overnight denture storage in addition to mechanical denture cleaning did not affect Candida albicans count, but reduced the total bacterial count on acrylic removable dentures compared to overnight storage in water. This effect was more pronounced when combined with ultrasonic cleaning compared to brushing.
ClinicalTrials.gov NCT02454413.
佩戴假牙者需要保持适当的口腔卫生以维护口腔健康。本研究旨在比较假牙清洁方法与过夜储存条件相结合对丙烯酸类可摘局部假牙上生物膜质量和成分的影响。
在一项针对13名老年人的交叉随机对照试验中，考虑了4种情况，包括2种不同的机械清洁方法和2种过夜储存条件：（i）刷牙并浸泡在不含清洁片的水中，（ii）刷牙并浸泡在含清洁片的水中，（iii）超声清洁并浸泡在不含清洁片的水中，以及（iv）超声清洁并浸泡在含清洁片的水中。每个测试条件连续进行5天，之前有2天的洗脱期。在基线（对照）和每个测试期结束时，从标准化区域采集生物膜样本。使用聚合酶链反应（PCR）技术鉴定选定的口腔细菌（n = 20）以及白色念珠菌的总数和个体水平。使用模拟假牙菌斑评分对假牙生物膜覆盖率进行评分。采用配对t检验和Wilcoxon符号秩检验比较测试条件。显著性水平设定为α<5%。
假牙在含清洁片的水中过夜储存可显著降低细菌总数（p<0.01）。两种机械清洁方法之间的细菌总数差异无统计学意义。未观察到对白色念珠菌数量或模拟菌斑评分有显著影响。
除了机械清洁假牙外，在假牙过夜储存期间使用清洁片不会影响白色念珠菌数量，但与在水中过夜储存相比，可减少丙烯酸类可摘局部假牙上的细菌总数。与刷牙相比，与超声清洁相结合时这种效果更明显。
ClinicalTrials.gov NCT02454413。
