Chamanzar Maysamreza, Garfield David J, Iafrati Jillian, Sohal Vikaas, Chan Emory, Cohen Bruce, Schuck P James, Maharbiz Michel M
Annu Int Conf IEEE Eng Med Biol Soc. 2015 Aug;2015:821-4. doi: 10.1109/EMBC.2015.7318488.
We demonstrate for the first time, the possibility of targeted optogenetic stimulation of neurons deep into brain tissue (>2 mm) in a minimally-invasive way by sending near-infrared light through tissue to excite passive lanthanide-doped blue-emitting upconverting nanocrystals (UCNPs) encapsulated in Parylene C microstructure light bulbs that emit visible (blue) light and locally excite opsins with high spatial resolution.
我们首次证明，通过组织传输近红外光以激发包裹在聚对二甲苯C微结构灯泡中的被动掺镧系元素的蓝色发光上转换纳米晶体（UCNPs），以微创方式对脑组织深处（>2 mm）的神经元进行靶向光遗传学刺激是可能的。这些纳米晶体发出可见光（蓝色），并以高空间分辨率局部激发视蛋白。
