Yang C H, Lambie E J, Hardin J, Craft J, Snyder M
Department of Biology, Yale University, New Haven, CT 06511.
Chromosoma. 1989 Aug;98(2):123-8. doi: 10.1007/BF00291048.
A panel of sera from 892 autoimmune patients was screened by indirect immunofluorescence on mammalian cells. Seventy-three sera were identified that recognize the nucleolus. Three of these sera appear to stain the nucleolus in yeast, suggesting that they recognize highly conserved antigens. These three sera also immunoprecipitate mammalian U3 snRNA-containing particles, which reside in the nucleolus and have been implicated in rRNA processing. Double immunofluorescence experiments with anti-nucleolus and anti-tubulin antibodies revealed a novel form of non-random nuclear organization in yeast. The spindle pole body and the nucleolus-both of which are associated with the nuclear envelope-preferentially localize at opposite ends of the nucleus. Organization of these and other components into specific regions of the nucleus may be important for optimizing their proper function.
用间接免疫荧光法在哺乳动物细胞上对892名自身免疫患者的一组血清进行筛选。鉴定出73份能识别核仁的血清。其中三份血清似乎能对酵母中的核仁进行染色，这表明它们识别的是高度保守的抗原。这三份血清还能免疫沉淀存在于核仁中且与rRNA加工有关的含哺乳动物U3 snRNA的颗粒。用抗核仁抗体和抗微管蛋白抗体进行的双重免疫荧光实验揭示了酵母中一种新的非随机核组织形式。纺锤体极体和核仁——两者都与核膜相关——优先定位在细胞核的相对两端。将这些及其他成分组织到细胞核的特定区域可能对优化其正常功能很重要。
