Andreas E M, Ambrosius H
Laboratory of Immunobiology, Karl Marx University, Leipzig, G.D.R.
Dev Comp Immunol. 1989 Spring;13(2):167-75. doi: 10.1016/0145-305x(89)90031-1.
Surface immunoglobulin bearing (sIg+) cells were identified in the tortoise Agrionemys horsfieldii by indirect immunofluorescence by using class-specific antisera to tortoise IgM and IgY produced in mice. With this technique 13-23% of blood lymphocytes were found to bear surface IgM and 5-8% IgY. Spleen lymphocytes show a higher percentage of sIg+ cells: 25-52% sIgM+ and 5-20% sIgY+ lymphocytes. There is strong indication that tortoise sIgY+ lymphocytes of blood and spleen also express IgM on their surface.
通过间接免疫荧光法,利用在小鼠体内产生的针对乌龟IgM和IgY的类特异性抗血清,在乌龟(Agrionemys horsfieldii)中鉴定出了表面带有免疫球蛋白(sIg+)的细胞。运用该技术发现,13%-23%的血液淋巴细胞带有表面IgM,5%-8%带有IgY。脾脏淋巴细胞显示出更高比例的sIg+细胞:25%-52%为sIgM+淋巴细胞,5%-20%为sIgY+淋巴细胞。有强有力的迹象表明,血液和脾脏中的乌龟sIgY+淋巴细胞在其表面也表达IgM。