粘质沙雷氏菌274鞭毛蛋白编码基因（hag）的克隆与核苷酸序列分析

Cloning and nucleotide sequence of a flagellin-coding gene (hag) from Serratia marcescens 274.

作者信息

Harshey R M, Estepa G, Yanagi H

机构信息

Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, CA 92037.

出版信息

Gene. 1989 Jun 30;79(1):1-8. doi: 10.1016/0378-1119(89)90087-5.

DOI:10.1016/0378-1119(89)90087-5

PMID:2673930

Abstract

The pigmented enterobacterium Serratia marcescens shows a high frequency of color variation. In strain 274, color variation was shown to be closely associated with flagellar variation [Paruchuri and Harshey, J. Bacteriol. 169 (1987) 61-65]. As a first step towards understanding the genetic basis of this phenomenon, we have cloned and sequenced a flagellin-coding gene of S. marcescens 274. The gene codes for a protein product of 351 aa, with a predicted Mr of 36,862. Amino acid sequence comparisons reveal a high degree of homology between the N-terminal half and C-terminal third of the Serratia flagellin and flagellins from Escherichia coli and Salmonella typhimurium.

摘要

产色素肠道杆菌粘质沙雷氏菌表现出很高的颜色变异频率。在274菌株中，颜色变异被证明与鞭毛变异密切相关[Paruchuri和Harshey，《细菌学杂志》169(1987)61 - 65]。作为理解这一现象遗传基础的第一步，我们克隆并测序了粘质沙雷氏菌274的鞭毛蛋白编码基因。该基因编码一个351个氨基酸的蛋白质产物，预测分子量为36,862。氨基酸序列比较显示，粘质沙雷氏菌鞭毛蛋白的N端一半和C端三分之一与大肠杆菌和鼠伤寒沙门氏菌的鞭毛蛋白之间具有高度同源性。