Jiang Wei-Wei, Liu Yu-Chen, Zhang Zhi-Jun, Liu Yan-Chun, He Juan, Su Jia, Cheng Xiao, Peng Li-Yan, Shao Li-Dong, Wu Xing-De, Yang Jia-Hui, Zhao Qin-Shi
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, PR China; College of Basic Science and Information Engineering, Yunnan Agricultura University, Kuming 6540201, PR China.
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, PR China.
Fitoterapia. 2016 Mar;109:155-61. doi: 10.1016/j.fitote.2015.12.017. Epub 2015 Dec 29.
Seven new fawcettimine-type (1-7) and two new lycopodine-type (8 and 9) Lycopodium alkaloids, as well as 10 known compounds, were isolated from the club moss, Lycopodium obscurum L. The structures of obscurumines H-P (1-9) were determined based on high-resolution MS and 1D and 2D NMR data. Compounds 1 and 2 include a new skeleton that is formed via the linkage of C-9-N-2', which is rarely present in Lycopodium alkaloids. The in vitro acetylcholinesterase (AChE) inhibitory activity assay showed that 5 exhibited weak anti-AChE activity with an IC50 value of 81.0 μM. Compound 8 exhibited inhibition of the secretion of IL-2 in phytohemagglutinin (PHA) and phorbol myristate acetate (PMA) stimulated Jurkat cells, and the IC50 value was 17.2 μM.
从石松属植物地刷子石松（Lycopodium obscurum L.）中分离出7种新的法氏石松碱型（1-7）和2种新的石松碱型（8和9）石松生物碱，以及10种已知化合物。基于高分辨率质谱和一维及二维核磁共振数据确定了暗石松碱H-P（1-9）的结构。化合物1和2包含一种新骨架，该骨架通过C-9-N-2'连接形成，这在石松生物碱中很少见。体外乙酰胆碱酯酶（AChE）抑制活性测定表明，5表现出较弱的抗AChE活性，IC50值为81.0 μM。化合物8在植物血凝素（PHA）和佛波酯（PMA）刺激的Jurkat细胞中表现出对白细胞介素-2分泌的抑制作用，IC50值为17.2 μM。
