Labeda David P
Mycotoxin Prevention and Applied Microbiology Research, National Center for Agricultural Utilization Research, USDA, Agricultural Research Service, 1815 N. University Street, Peoria, IL, 61604, USA.
Antonie Van Leeuwenhoek. 2016 Mar;109(3):349-56. doi: 10.1007/s10482-015-0637-6. Epub 2016 Jan 6.
Multi-locus sequence analysis has been demonstrated to be a useful tool for identification of Streptomyces species and was previously applied to phylogenetically differentiate the type strains of species pathogenic on potatoes (Solanum tuberosum L.). The ARS Culture Collection (NRRL) contains 43 strains identified as Streptomyces scabiei deposited at various times since the 1950s and these were subjected to multi-locus sequence analysis utilising partial sequences of the house-keeping genes atpD, gyrB, recA, rpoB and trpB. Phylogenetic analyses confirmed the identity of 17 of these strains as Streptomyces scabiei, 9 of the strains as the potato-pathogenic species Streptomyces europaeiscabiei and 6 strains as potentially new phytopathogenic species. Of the 16 other strains, 12 were identified as members of previously described non-pathogenic Streptomyces species while the remaining 4 strains may represent heretofore unrecognised non-pathogenic species. This study demonstrated the value of this technique for the relatively rapid, simple and sensitive molecular identification of Streptomyces strains held in culture collections.
多位点序列分析已被证明是鉴定链霉菌属物种的一种有用工具，并且此前已应用于从系统发育角度区分对马铃薯（Solanum tuberosum L.）致病的物种的模式菌株。ARS菌种保藏中心（NRRL）包含自20世纪50年代以来不同时间保存的43株被鉴定为疮痂链霉菌的菌株，利用看家基因atpD、gyrB、recA、rpoB和trpB的部分序列对这些菌株进行了多位点序列分析。系统发育分析证实，其中17株菌株为疮痂链霉菌，9株为马铃薯致病物种欧洲疮痂链霉菌，6株为潜在的新植物致病物种。在其他16株菌株中，12株被鉴定为先前描述的非致病链霉菌属物种的成员，其余4株菌株可能代表迄今未被认识的非致病物种。这项研究证明了该技术对于培养物保藏中链霉菌菌株相对快速、简单和灵敏的分子鉴定的价值。
