Idris Hamidah, Labeda David P, Nouioui Imen, Castro Jean Franco, Del Carmen Montero-Calasanz Maria, Bull Alan T, Asenjo Juan A, Goodfellow Michael
School of Biology, Newcastle University, Ridley Building 2, Newcastle upon Tyne, NE1 7RU, UK.
National Center for Agricultural Utilization Research, USDA-ARS, Peoria, IL, 61604, USA.
Antonie Van Leeuwenhoek. 2017 May;110(5):705-717. doi: 10.1007/s10482-017-0838-2. Epub 2017 Feb 9.
A polyphasic study was undertaken to determine the taxonomic status of a Streptomyces strain which had been isolated from a high altitude Atacama Desert soil and shown to have bioactive properties. The strain, isolate H9, was found to have chemotaxonomic, cultural and morphological properties that place it in the genus Streptomyces. 16S rRNA gene sequence analyses showed that the isolate forms a distinct branch at the periphery of a well-delineated subclade in the Streptomyces 16S rRNA gene tree together with the type strains of Streptomyces crystallinus, Streptomyces melanogenes and Streptomyces noboritoensis. Multi-locus sequence analysis (MLSA) based on five house-keeping gene alleles showed that isolate H9 is closely related to the latter two type strains and to Streptomyces polyantibioticus NRRL B-24448. The isolate was distinguished readily from the type strains of S. melanogenes, S. noboritoensis and S. polyantibioticus using a combination of phenotypic properties. Consequently, the isolate is considered to represent a new species of Streptomyces for which the name Streptomyces aridus sp. nov. is proposed; the type strain is H9 (=NCIMB 14965=NRRL B65268). In addition, the MLSA and phenotypic data show that the S. melanogenes and S. noboritoensis type strains belong to a single species, it is proposed that S. melanogenes be recognised as a heterotypic synonym of S. noboritoensis for which an emended description is given.
开展了一项多阶段研究，以确定从高海拔阿塔卡马沙漠土壤中分离出的一株具有生物活性的链霉菌菌株的分类地位。该菌株（分离株H9）具有化学分类学、培养和形态学特性，将其归入链霉菌属。16S rRNA基因序列分析表明，该分离株在链霉菌16S rRNA基因树中一个明确界定的亚分支外围形成一个独特分支，与结晶链霉菌、产黑素链霉菌和野堀链霉菌的模式菌株在一起。基于五个管家基因等位基因的多位点序列分析（MLSA）表明，分离株H9与后两个模式菌株以及多抗生素链霉菌NRRL B-24448密切相关。利用表型特性组合，该分离株很容易与产黑素链霉菌、野堀链霉菌和多抗生素链霉菌的模式菌株区分开来。因此，该分离株被认为代表链霉菌属的一个新物种，为此提出名称干旱链霉菌新种；模式菌株为H9（=NCIMB 14965=NRRL B65268）。此外，MLSA和表型数据表明，产黑素链霉菌和野堀链霉菌模式菌株属于同一个物种，建议将产黑素链霉菌视为野堀链霉菌的异名，并给出修订后的描述。
