AbdulQader Sarah T, Rahman Ismail A, Thirumulu Kannan P, Ismail Hanafi, Mahmood Zuliani
School of Dental Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia Department of Pedodontic and Preventive Dentistry, College of Dentistry, University of Baghdad, Baghdad, Iraq.
School of Dental Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia.
J Biomater Appl. 2016 Apr;30(9):1300-11. doi: 10.1177/0885328215625759. Epub 2016 Jan 5.
Calcium phosphates (CaP) of different porosities have been widely and successfully used as scaffolds with osteoblast cells for bone tissue regeneration. However, the effects of scaffold porosities on cell viability and differentiation of human dental pulp cells for dentin tissue regeneration are not well known. In this study, biphasic calcium phosphate (BCP) scaffolds of 20/80 hydroxyapatite to beta tricalcium phosphate ratio with a mean pore size of 300 μm were prepared into BCP1, BCP2, BCP3, and BCP4 of 25%, 50%, 65%, and 75% of total porosities, respectively. The extracts of these scaffolds were assessed with regard to cell viability, proliferation, and differentiation of human dental pulp cells. The high alkalinity, and more calcium and phosphate ions release that were exhibited by BCP3 and BCP4 decreased the viability and proliferation of human dental pulp cells as compared to BCP1 and BCP2. BCP2 significantly increased both cell viability and cell proliferation. However, the cells cultured with BCP3 extract revealed high alkaline phosphatase (ALP) activity and high expression of odontoblast related genes, collagen type I alpha 1, dentin matrix protein-1, and dentin sialophosphoprotein as compared to that cultured with BCP1, BCP2, and BCP4 extracts. The results highlight the effect of different scaffold porosities on the cell microenvironment and demonstrate that BCP3 scaffold of 65% porosity can support human dental pulp cells differentiation for dentin tissue regeneration.
不同孔隙率的磷酸钙（CaP）已被广泛且成功地用作成骨细胞支架用于骨组织再生。然而，支架孔隙率对人牙髓细胞活力和牙本质组织再生分化的影响尚不明确。在本研究中，将羟基磷灰石与β-磷酸三钙比例为20/80、平均孔径为300μm的双相磷酸钙（BCP）支架分别制备成总孔隙率为25%、50%、65%和75%的BCP1、BCP2、BCP3和BCP4。评估了这些支架提取物对人牙髓细胞活力、增殖和分化的影响。与BCP1和BCP2相比，BCP3和BCP4表现出的高碱度以及更多的钙和磷酸根离子释放降低了人牙髓细胞的活力和增殖。BCP2显著提高了细胞活力和细胞增殖。然而，与用BCP1、BCP2和BCP4提取物培养的细胞相比，用BCP3提取物培养的细胞显示出高碱性磷酸酶（ALP）活性以及成牙本质细胞相关基因、I型胶原蛋白α1、牙本质基质蛋白-1和牙本质涎磷蛋白的高表达。结果突出了不同支架孔隙率对细胞微环境的影响，并表明孔隙率为65%的BCP3支架可支持人牙髓细胞向牙本质组织再生方向分化。
