Xu Jiayu, Shao Xingfeng, Li Yonghua, Wei Yingying, Xu Feng, Wang Hongfei
Department of Food Science and Engineering, Ningbo UniversityNingbo, China.
Front Microbiol. 2017 Jun 6;8:1017. doi: 10.3389/fmicb.2017.01017. eCollection 2017.
Tea tree oil (TTO), a volatile essential oil, has been widely used as an antimicrobial agent. However, the mechanism underlying TTO antifungal activity is not fully understood. In this study, a comprehensive metabolomics survey was undertaken to identify changes in metabolite production in cells treated with TTO. Significant differences in 91 metabolites were observed, including 8 upregulated and 83 downregulated metabolites in TTO-treated cells. The results indicate that TTO inhibits primary metabolic pathways through the suppression of the tricarboxylic acid (TCA) cycle and fatty acid metabolism. Further experiments show that TTO treatment decreases the activities of key enzymes in the TCA cycle and increases the level of hydrogen peroxide (HO). Membrane damage is also induced by TTO treatment. We hypothesize that the effect of TTO on is achieved mainly by disruption of the TCA cycle and fatty acid metabolism, resulting in mitochondrial dysfunction and oxidative stress.
茶树油（TTO）是一种挥发性精油，已被广泛用作抗菌剂。然而，TTO抗真菌活性的潜在机制尚未完全明确。在本研究中，进行了全面的代谢组学调查，以确定用TTO处理的细胞中代谢产物产生的变化。观察到91种代谢产物存在显著差异，包括TTO处理的细胞中有8种上调代谢产物和83种下调代谢产物。结果表明，TTO通过抑制三羧酸（TCA）循环和脂肪酸代谢来抑制初级代谢途径。进一步的实验表明，TTO处理会降低TCA循环中关键酶的活性，并增加过氧化氢（HO）水平。TTO处理还会诱导膜损伤。我们推测，TTO对[此处原文缺失相关内容]的作用主要是通过破坏TCA循环和脂肪酸代谢来实现的，从而导致线粒体功能障碍和氧化应激。
