Priyantha Roshan, Gaunt Mathew C, Rubin Joseph E
Department of Veterinary Microbiology (Priyantha, Rubin), Department of Small Animal Clinical Sciences (Gaunt), Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada.
Can Vet J. 2016 Jan;57(1):65-9.
This study reports antimicrobial susceptibility of Staphylococcus pseudintermedius carried by healthy dogs in Saskatoon, and describes changes in antimicrobial resistance since a 2008 study. One hundred healthy dogs presenting to the wellness service at the Western College of Veterinary Medicine were screened for S. pseudintermedius by culturing rectal and pharyngeal swabs. Staphylococcus pseudintermedius was identified biochemically and antimicrobial minimum inhibitory concentrations were determined by broth microdilution. Methicillin resistance was confirmed by polymerase chain reaction (PCR) and sequencing of the mecA gene. Of 221 S. pseudintermedius isolates from 78 dogs, 7 were methicillin resistant. No resistance to the fluoroquinolones, nitrofurantoin, tigecycline, vancomycin, quinupristin-dalfopristin, linezolid, or daptomycin was identified. Of the 78 positive dogs, isolates resistant to penicillin were found in 78%, to ampicillin in 61% and to tetracycline in 26%; resistance to oxacillin, erythromycin, clindamycin, trimethoprim + sulfamethoxazole, chloramphenicol, and gentamicin was found in < 10% of dogs. Compared to the 2008 study, the frequency of resistance to all drugs increased, and the frequency of colonization with pan-susceptible isolates decreased from 46% to 30%.
本研究报告了萨斯卡通市健康犬所携带的中间型假葡萄球菌的抗菌药敏情况，并描述了自2008年研究以来抗菌耐药性的变化。对前往西部兽医学院健康服务中心的100只健康犬进行了筛查，通过培养直肠和咽拭子来检测中间型假葡萄球菌。通过生化方法鉴定中间型假葡萄球菌，并采用肉汤微量稀释法测定抗菌药物的最低抑菌浓度。通过聚合酶链反应（PCR）和mecA基因测序确认耐甲氧西林情况。在从78只犬分离出的221株中间型假葡萄球菌中，有7株耐甲氧西林。未发现对氟喹诺酮类、呋喃妥因、替加环素、万古霉素、奎奴普丁-达福普汀、利奈唑胺或达托霉素耐药。在78只阳性犬中，78%的犬分离株对青霉素耐药，61%对氨苄西林耐药，26%对四环素耐药；对苯唑西林、红霉素、克林霉素、甲氧苄啶+磺胺甲恶唑、氯霉素和庆大霉素耐药的犬不到10%。与2008年的研究相比，所有药物的耐药频率均有所增加，全敏感分离株的定植频率从46%降至30%。
