Key Laboratory for Biorheological Science and Technology of Ministry of Education (Chongqing University), State and Local Joint Engineering Laboratory for Vascular Implants (Chongqing), Bioengineering College of Chongqing University, Chongqing University, Chongqing, China.
Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, College of Urban Construction and Environmental Engineering, Chongqing University, Chongqing, China.
Environ Sci Pollut Res Int. 2016 Apr;23(8):7639-47. doi: 10.1007/s11356-015-6024-z. Epub 2016 Jan 8.
The aim of this study was to judge the legal duty of pollution liabilities by assessing a duck faeces-specific marker, which can exclude distractions of residual bacteria from earlier contamination accidents. With the gene sequencing technology and bioinformatics method, we completed the comparative analysis of Faecalibacterium sequences, which were associated with ducks and other animal species, and found the sequences unique to duck faeces. Polymerase chain reaction (PCR) and agarose gel electrophoresis techniques were used to verify the reliability of both human and duck faeces-specific primers. The duck faeces-specific primers generated an amplicon of 141 bp from 43.3 % of duck faecal samples, 0 % of control samples and 100 % of sewage wastewater samples that contained duck faeces. We present here the initial evidence of Faecalibacterium-based applicability as human faeces-specificity in China. Meanwhile, this study represents the initial report of a Faecalibacterium marker for duck faeces and suggests an independent or supplementary environmental biotechnology of microbial source tracking (MST).
本研究旨在通过评估一种鸭粪便特异性标志物来判断污染责任的法律义务，该标志物可以排除早期污染事故中残留细菌的干扰。我们利用基因测序技术和生物信息学方法，完成了与鸭和其他动物物种相关的 Faecalibacterium 序列的比较分析，发现了鸭粪便特有的序列。聚合酶链反应（PCR）和琼脂糖凝胶电泳技术用于验证人和鸭粪便特异性引物的可靠性。鸭粪便特异性引物从 43.3%的鸭粪便样本中产生了 141bp 的扩增子，0%的对照样本和 100%的含有鸭粪便的污水废水样本中均未产生扩增子。本研究初步证实了 Faecalibacterium 在我国作为人粪便特异性的适用性。同时，本研究首次报道了一种用于鸭粪便的 Faecalibacterium 标志物，并提出了一种独立或补充的微生物源追踪（MST）环境生物技术。
