a Interdepartmental Genetics and Genomics Program; Iowa State University ; Ames , IA USA.
b Department of Agronomy ; Iowa State University ; Ames , IA USA.
GM Crops Food. 2015;6(4):266-76. doi: 10.1080/21645698.2015.1137690.
Targeted genome editing is now possible in nearly any organism and is widely acknowledged as a biotech game-changer. Among available gene editing techniques, the CRISPR-Cas9 system is the current favorite because it has been shown to work in many species, does not necessarily result in the addition of foreign DNA at the target site, and follows a set of simple design rules for target selection. Use of the CRISPR-Cas9 system is facilitated by the availability of an array of CRISPR design tools that vary in design specifications and parameter choices, available genomes, graphical visualization, and downstream analysis functionality. To help researchers choose a tool that best suits their specific research needs, we review the functionality of various CRISPR design tools including our own, the CRISPR Genome Analysis Tool (CGAT; http://cropbioengineering.iastate.edu/cgat ).
靶向基因组编辑现在几乎可以在任何生物体中进行，被广泛认为是生物技术的游戏规则改变者。在现有的基因编辑技术中，CRISPR-Cas9 系统是目前的首选，因为它已经在许多物种中得到证实，并且不一定会在目标位点添加外源 DNA，而且遵循一套简单的目标选择设计规则。CRISPR-Cas9 系统的使用得益于一系列 CRISPR 设计工具的可用性，这些工具在设计规格和参数选择、可用基因组、图形可视化和下游分析功能方面有所不同。为了帮助研究人员选择最适合他们特定研究需求的工具，我们回顾了各种 CRISPR 设计工具的功能，包括我们自己的 CRISPR 基因组分析工具 (CGAT；http://cropbioengineering.iastate.edu/cgat)。
