Wong Nathan, Liu Weijun, Wang Xiaowei
Department of Biomedical Engineering, Washington University, St Louis, MO, 63130, USA.
Department of Radiation Oncology, Washington University School of Medicine, St Louis, MO, 63108, USA.
Genome Biol. 2015 Nov 2;16:218. doi: 10.1186/s13059-015-0784-0.
The CRISPR/Cas9 system has been rapidly adopted for genome editing. However, one major issue with this system is the lack of robust bioinformatics tools for design of single guide RNA (sgRNA), which determines the efficacy and specificity of genome editing. To address this pressing need, we analyze CRISPR RNA-seq data and identify many novel features that are characteristic of highly potent sgRNAs. These features are used to develop a bioinformatics tool for genome-wide design of sgRNAs with improved efficiency. These sgRNAs as well as the design tool are freely accessible via a web server, WU-CRISPR ( http://crispr.wustl.edu ).
CRISPR/Cas9系统已被迅速应用于基因组编辑。然而，该系统的一个主要问题是缺乏用于设计单向导RNA（sgRNA）的强大生物信息学工具，而sgRNA决定了基因组编辑的效率和特异性。为满足这一迫切需求，我们分析了CRISPR RNA测序数据，并识别出许多高效sgRNA所特有的新特征。这些特征被用于开发一种生物信息学工具，以提高全基因组sgRNA设计的效率。这些sgRNA以及设计工具可通过网络服务器WU-CRISPR（http://crispr.wustl.edu）免费获取。
Zotero 插件
