Rosnina A G, Tan Yee Shin, Abdullah Noorlidah, Vikineswary S
Mushroom Research Centre, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia.
Department of Agroecotechnology of Agriculture Faculty, University of Malikussaleh, Lhokseumawe, Aceh, Indonesia.
World J Microbiol Biotechnol. 2016 Feb;32(2):18. doi: 10.1007/s11274-015-1959-2. Epub 2016 Jan 8.
Pleurotus citrinopileatus (yellow oyster mushroom) has an attractive shape and yellow colour but the fragile texture complicates packaging, and its strong aroma is unappealing to consumers. This study aimed to improve the characteristics and yield of P. citrinopileatus by interspecies mating between monokaryotic cultures of P. citrinopileatus and P. pulmonarius. Ten monokaryon cultures of the parental lines were crossed in all combinations to obtain hybrids. Eleven compatible mating pairs were obtained and cultivated to observe their sporophore morphology and yield. The selected hybrid, i.e. P1xC9, was beige in colour while hybrid P3xC8 was yellow in colour. Their sporophores had less offensive aroma, improved texture and higher yield. The DNA sequences of these hybrids were found to be in the same clade as the P. citrinopileatus parent with a bootstrap value of 99%. High bootstrap values indicate high genetic homology between hybrids and the P. citrinopileatus parent. The biological efficiencies of these hybrids P1xC9 (70.97%) and P3xC8 (52.14%) were also higher than the P. citrinopileatus parent (35.63%). Interspecies hybrids obtained by this mating technique can lead to better strains of mushrooms for genetic improvement of the Pleurotus species.
金顶侧耳（金顶菇）外形诱人且呈黄色，但质地脆弱给包装带来困难，其强烈气味也不受消费者欢迎。本研究旨在通过金顶侧耳与肺形侧耳单核培养物之间的种间杂交来改善金顶侧耳的特性和产量。将亲本系的10个单核培养物进行所有组合的杂交以获得杂种。获得了11个相容的交配组合并进行培养，以观察其子实体形态和产量。所选杂种，即P1xC9，呈米色，而杂种P3xC8呈黄色。它们的子实体气味较淡、质地改善且产量更高。发现这些杂种的DNA序列与金顶侧耳亲本处于同一进化枝，自展值为99%。高自展值表明杂种与金顶侧耳亲本之间具有高度的遗传同源性。这些杂种P1xC9（70.97%）和P3xC8（52.14%）的生物学效率也高于金顶侧耳亲本（35.63%）。通过这种交配技术获得的种间杂种可培育出更好的蘑菇菌株，用于侧耳属物种的遗传改良。
