Shnyreva A A, Sivolapova A B, Shnyreva A V
Genetika. 2012 Nov;48(11):1260-70.
Two closely related commercially cultivated oyster mushroom species, Pleurotus pulmonarius and P. sajor-caju have been differentiated by traditional mating experiments as well as analysis of the variable ITS and IGS sequences of the ribosomal gene cluster. Molecular analysis of the variable ITS and IGS regions has allowed neither reliable differentiation between the morphologically similar species P. pulmonarius and P. sajor-caju nor confirmation of species identity of the P. sajor-caju strains CS-32, H-1, and H-2. Analysis of the sexual (mating) compatibility between haploid tester strains of these two species in monokaryon-monokaryon mating experiments has demonstrated complete reproductive isolation between P. pulmonarius and P. sajor-caju, thereby confirming that these are separate species.
两种密切相关的商业栽培平菇品种，肺形侧耳（Pleurotus pulmonarius）和佛罗里达侧耳（P. sajor-caju），已通过传统的交配实验以及核糖体基因簇可变ITS和IGS序列分析得以区分。对可变ITS和IGS区域的分子分析既无法可靠地区分形态相似的肺形侧耳和佛罗里达侧耳品种，也无法确认佛罗里达侧耳菌株CS-32、H-1和H-2的品种身份。在单核体-单核体交配实验中对这两个品种的单倍体测试菌株之间的有性（交配）相容性分析表明，肺形侧耳和佛罗里达侧耳之间存在完全的生殖隔离，从而证实它们是不同的品种。
