Arantes Eva L, Dragano Nathalia, Ramalho Albina, Vitorino Daniele, de-Souza Gabriela F, Lima Maria H M, Velloso Licio A, Araújo Eliana P
Nursing School, University of Campinas, Campinas SP, Brazil.
Laboratory of Cell Signaling, University of Campinas, Campinas SP, Brazil.
Biol Res Nurs. 2016 Jul;18(4):411-9. doi: 10.1177/1099800415621617. Epub 2016 Jan 8.
The development of methods for improving skin wound healing may have an impact on the outcomes of a number of medical conditions. The topical use of polyunsaturated fatty acids (PUFAs) can accelerate skin wound healing through mechanisms that involve, at least in part, the modulation of inflammatory activity.
We evaluated whether G-protein-coupled receptor 120 (GPR120), a recently identified receptor for docosahexaenoic acid (DHA) with anti-inflammatory activity, is expressed in the skin and responds to topical DHA.
Male Wistar rats were submitted to an 8.0-mm wound on the back and were immediately administered a topical treatment of a solution containing 30 μM of DHA once a day. The healing process was photodocumented, and tissues were collected on Days 5, 9, and 15 for protein and RNA analyses and histological evaluation.
GPR120 was expressed in the intact skin and in the wound. Keratinocytes expressed the most skin GPR120, while virtually no expression was detected in fibroblasts. Upon DHA topical treatment, wound healing was significantly accelerated and was accompanied by the molecular activation of GPR120, as determined by its association with β-arrestin-2. In addition, DHA promoted a reduction in the expression of interleukin (IL) 1β and an increase in the expression of IL-6. Furthermore, there was a significant increase in expression of transforming growth factor β (TGF-β) and the keratinocyte marker involucrin.
Topical DHA improved skin wound healing. The activation of GPR120 is potentially involved in this process.
改进皮肤伤口愈合方法的发展可能会对许多医疗状况的结果产生影响。多不饱和脂肪酸（PUFAs）的局部应用可通过至少部分涉及炎症活动调节的机制加速皮肤伤口愈合。
我们评估了G蛋白偶联受体120（GPR120），一种最近发现的具有抗炎活性的二十二碳六烯酸（DHA）受体，是否在皮肤中表达并对局部应用的DHA作出反应。
雄性Wistar大鼠背部制造一个8.0毫米的伤口，并立即每天一次局部给予含30μM DHA的溶液治疗。愈合过程进行拍照记录，在第5、9和15天收集组织进行蛋白质和RNA分析以及组织学评估。
GPR120在完整皮肤和伤口中均有表达。角质形成细胞表达皮肤中最多的GPR120，而成纤维细胞中几乎未检测到表达。局部应用DHA后，伤口愈合明显加速，并伴有GPR120的分子激活，这通过其与β-抑制蛋白-2的结合来确定。此外，DHA促进白细胞介素（IL）1β表达降低和IL-6表达增加。此外，转化生长因子β（TGF-β）和角质形成细胞标志物兜甲蛋白的表达显著增加。
局部应用DHA改善了皮肤伤口愈合。GPR120的激活可能参与了这一过程。
