Bassel George W, Smith Richard S
School of Biosciences, University of Birmingham, Birmingham B15 2TT, UK.
Department of Comparative Development and Genetics, Max Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, 50829 Cologne, Germany.
Curr Opin Plant Biol. 2016 Feb;29:87-94. doi: 10.1016/j.pbi.2015.11.005. Epub 2016 Jan 1.
Plant development occurs in 3D space over time (4D). Recent advances in image acquisition and computational analysis are now enabling development to be visualized and quantified in its entirety at the cellular level. The simultaneous quantification of reporter abundance and 3D cell shape change enables links between signaling processes and organ morphogenesis to be accomplished organ-wide and at single cell resolution. Current work to integrate this quantitative 3D image data with computational models is enabling causal relationships between gene expression and organ morphogenesis to be uncovered. Further technical advances in imaging and image analysis will enable this approach to be applied to a greater diversity of plant organs and will become a key tool to address many questions in plant development.
植物发育随时间在三维空间（四维）中发生。图像采集和计算分析方面的最新进展使得能够在细胞水平上对发育进行整体可视化和量化。对报告基因丰度和三维细胞形状变化的同时量化，使得能够在器官范围内以单细胞分辨率完成信号传导过程与器官形态发生之间的关联。目前将这种定量三维图像数据与计算模型相结合的工作，能够揭示基因表达与器官形态发生之间的因果关系。成像和图像分析方面的进一步技术进步将使这种方法能够应用于更多种类的植物器官，并将成为解决植物发育中许多问题的关键工具。
