Yang Song-Yi, Kang Min-Kyung
Department and Research Institute of Dental Biomaterials and Bioengineering, Yonsei University College of Dentistry, Seoul 03722, Korea.
Department of Dental Hygiene, Hanseo University, Chungcheongnam-do 31962, Korea.
Plants (Basel). 2020 Sep 29;9(10):1292. doi: 10.3390/plants9101292.
We conducted surface characterization to assess the biocompatibility and investigate the antimicrobial activity against oral pathogens in autopolymerizing acrylic resins, coated with light-curable coating resin, containing various concentrations of extract (0, 200, 400, and 600 µg/mL). The extract powder was prepared using a freeze-drying technique. Further, a goniometer and microhardness tester were used to determine the water contact angle, and Vickers hardness, respectively; color measurements were performed on the uncoated and coated acrylic resin disks. The polyphenol content of the extracts from the coated acrylic resin disk was analyzed using UV-VIS spectroscopy. The antimicrobial activity of the coated acrylic resin disk against and was observed for 24 and 48 h by measuring the optical density using spectrophotometry. In addition, biocompatibility was confirmed by testing the cell viability according to ISO 10993-5. The water contact angle, Vickers hardness, and color change values of the coated acrylic resin disks were not significantly different from the control. Polyphenol was detected in all experimental groups, with no significant differences between the experimental groups. The experimental groups exhibited significant antimicrobial activity against and compared to the control group, after 48 h of incubation. The cell viability between the control and experimental groups was not significantly different. The proposed coating resin containing extract is applicable on dental acrylic resins, due to their antimicrobial properties and excellent biocompatibility, with no deterioration of surface characteristics.
我们进行了表面表征，以评估生物相容性，并研究含有不同浓度提取物（0、200、400和600 µg/mL）的、涂有光固化涂层树脂的自聚合丙烯酸树脂对口腔病原体的抗菌活性。提取物粉末采用冷冻干燥技术制备。此外，分别使用测角仪和显微硬度计测定水接触角和维氏硬度；对未涂层和涂层丙烯酸树脂圆盘进行颜色测量。使用紫外可见光谱法分析涂层丙烯酸树脂圆盘提取物中的多酚含量。通过分光光度法测量光密度，观察涂层丙烯酸树脂圆盘对[具体菌种1]和[具体菌种2]的抗菌活性24小时和48小时。此外，根据ISO 10993-5测试细胞活力，确认了生物相容性。涂层丙烯酸树脂圆盘的水接触角、维氏硬度和颜色变化值与对照组无显著差异。所有实验组均检测到多酚，各实验组之间无显著差异。孵育48小时后，实验组对[具体菌种1]和[具体菌种2]的抗菌活性明显高于对照组。对照组和实验组之间的细胞活力无显著差异。所提出的含有提取物的涂层树脂因其抗菌性能和优异的生物相容性，且不会使表面特性恶化，适用于牙科丙烯酸树脂。
