Om Ae-Son, Song Yu-Na, Noh GeonMin, Kim HaengRan, Choe JeongSook
Laboratory of Food Safety and Toxicology, Department of Food Science and Nutrition, Hanyang University, Seoul 133-791, Korea.
Functional Food and Nutrition Division, Department of Agrofood Resources, National Academy of Agricultural Science, Jeonbuk 565-851, Korea.
Molecules. 2016 Jan 8;21(1):65. doi: 10.3390/molecules21010065.
The leaves and stems of the plant Rubus coreanus Miquel (RCMLS) are rich in vitamins, minerals and phytochemicals which have antioxidant, anti-hemolytic, anti-inflammatory, anti-fatigue and anti-cancer effects. However, RCMLS is not included in the Korean Food Standards Codex due to the lack of safety assurance concerning RCMLS. We evaluated single and repeated oral dose toxicity of RCMLS in Sprague-Dawley rats. RCMLS did not induce any significant toxicological changes in both male and female rats at a single doses of 2500 mg/kg/day. Repeated oral dose toxicity studies showed no adverse effects in clinical signs, body weight, food consumption, ophthalmic examination, urinalysis, hematology, serum biochemistry, necropsy findings, organ weight, and histopathology at doses of 625, 1250, and 2500 mg/kg/day. The LD50 and LOAEL of RCMLS might be over 2500 mg/kg body weight/day and no target organs were identified. Therefore, this study revealed that single and repeated oral doses of RCMLS are safe.
悬钩子（Rubus coreanus Miquel，RCMLS）的叶和茎富含维生素、矿物质和植物化学物质，具有抗氧化、抗溶血、抗炎、抗疲劳和抗癌作用。然而，由于缺乏关于RCMLS的安全性保证，RCMLS未被列入韩国食品标准法典。我们评估了RCMLS对Sprague-Dawley大鼠的单次和重复口服剂量毒性。RCMLS在单次剂量为2500 mg/kg/天时，对雄性和雌性大鼠均未引起任何显著的毒理学变化。重复口服剂量毒性研究表明，在剂量为625、1250和2500 mg/kg/天时，RCMLS在临床体征、体重、食物消耗、眼科检查、尿液分析、血液学、血清生物化学、尸检结果、器官重量和组织病理学方面均无不良影响。RCMLS的半数致死剂量（LD50）和最低观察到有害作用剂量（LOAEL）可能超过2500 mg/kg体重/天，且未发现靶器官。因此，本研究表明，RCMLS的单次和重复口服剂量是安全的。
