Zaman Sayed, Yazdani Umar, Deng Yan, Li Wenhao, Gadad Bharathi S, Hynan Linda, Karp David, Roatch Nichole, Schutte Claire, Nathan Marti C, Hewitson Laura, German Dwight C
Department of Psychiatry, UT Southwestern Medical Center, Dallas TX.
Department of Clinical Sciences, UT Southwestern Medical Center, Dallas TX.
Sci Rep. 2016 Jan 14;6:19164. doi: 10.1038/srep19164.
Autism spectrum disorder (ASD) is a neurodevelopmental disorder characterized by impairments in social interaction and communication, and restricted, repetitive patterns of behavior. In order to identify individuals with ASD and initiate interventions at the earliest possible age, biomarkers for the disorder are desirable. Research findings have identified widespread changes in the immune system in children with autism, at both systemic and cellular levels. In an attempt to find candidate antibody biomarkers for ASD, highly complex libraries of peptoids (oligo-N-substituted glycines) were screened for compounds that preferentially bind IgG from boys with ASD over typically developing (TD) boys. Unexpectedly, many peptoids were identified that preferentially bound IgG from TD boys. One of these peptoids was studied further and found to bind significantly higher levels (>2-fold) of the IgG1 subtype in serum from TD boys (n = 60) compared to ASD boys (n = 74), as well as compared to older adult males (n = 53). Together these data suggest that ASD boys have reduced levels (>50%) of an IgG1 antibody, which resembles the level found normally with advanced age. In this discovery study, the ASD1 peptoid was 66% accurate in predicting ASD.
自闭症谱系障碍（ASD）是一种神经发育障碍，其特征是社交互动和沟通受损，以及行为受限、重复。为了识别患有ASD的个体并尽早开始干预，该疾病的生物标志物是很有必要的。研究结果已确定自闭症儿童的免疫系统在全身和细胞水平上都有广泛变化。为了寻找ASD的候选抗体生物标志物，对高度复杂的类肽（寡聚N-取代甘氨酸）文库进行了筛选，以寻找优先结合ASD男孩而非发育正常（TD）男孩的IgG的化合物。出乎意料的是，发现许多类肽优先结合TD男孩的IgG。对其中一种类肽进行了进一步研究，发现与ASD男孩（n = 74）以及老年男性（n = 53）相比，它与TD男孩（n = 60）血清中IgG1亚型的结合水平显著更高（>2倍）。这些数据共同表明，ASD男孩的一种IgG1抗体水平降低（>50%），这类似于正常情况下老年时的水平。在这项发现研究中，ASD1类肽在预测ASD方面的准确率为66%。
