Wei Mei, Du Lan-zhe, Li Hui, Zhang Guang-da, Chen Xiang-dong
Zhong Yao Cai. 2015 May;38(5):1066-9.
To study the correlation of characteristic spectra of Vinegar Corydalis Rhizoma decoction pieces, water decoction and formula granules by HPLC, and to investigate the transfer of the main chemical constituents between three different forms.
The analysis was carried out by a Phenomenex Gemini C18 column (250 mm x 4.6 mm,5 μm) with acetonitrile-1% acetic acid and ammonium acetate buffer solution (pH 6.0) as the mobile phase in a gradient elution mode. The detection wavelength was 280 nm with a flow rate of 0.8 mL /min. The column temperature was 30 degrees C. The characteristic spectra from 11 batches of Vinegar Corydalis Rhizoma decoction pieces, 11 batches of water decoction and 11 batches of formula granules were established respectively.
Ten peaks in the HPLC characteristic spectra from 11 batches of formula granules could be tracked in the water decoction, nine peaks in the HPLC characteristic spectra could be tracked in the decoction pieces. In the ten common peaks, four components such as protopine, palnatine chloride, berberine hydrochloride and tetrahydropalmatine were verified.
The main chemical components of Vinegar Corydalis Rhizoma decoction pieces, water decoction and formula granules are basically the same, the common component contents have similar proportion.
采用高效液相色谱法研究醋延胡索饮片、水煎液及配方颗粒特征图谱的相关性，考察3种不同剂型中主要化学成分的转移情况。
采用菲罗门Gemini C18色谱柱（250 mm×4.6 mm，5μm），以乙腈-1%醋酸和醋酸铵缓冲液（pH 6.0）为流动相，梯度洗脱。检测波长为280 nm，流速为0.8 mL/min。柱温为30℃。分别建立11批次醋延胡索饮片、11批次水煎液及11批次配方颗粒的特征图谱。
11批次配方颗粒的高效液相色谱特征图谱中有10个峰可在水煎液中追踪到，高效液相色谱特征图谱中有9个峰可在饮片中追踪到。在10个共有峰中，鉴定出原阿片碱、氯化巴马汀、盐酸小檗碱、延胡索乙素4种成分。
醋延胡索饮片、水煎液及配方颗粒的主要化学成分基本相同，共有成分含量比例相近。
